Thakkar Jinal B, Aghera Dimpal J, Trivedi Bhavana, Prabha C Ratna
Department of Biochemistry, Faculty of Science, The M. S. University of Baroda, Vadodara 390005, India.
Department of Chemistry, Faculty of Science, The M. S. University of Baroda, Vadodara 390005, India.
Int J Biol Macromol. 2023 Feb 28;229:136-145. doi: 10.1016/j.ijbiomac.2022.12.281. Epub 2022 Dec 28.
High levels of triglycerides in blood can harden and block the arteries increasing the risk of heart disease and strokes. Triglycerides are important constituents of oils and fats used in various foods. The triglyceride content in commercial preparations of oils is estimated using conventional methods. In the present study, an electrochemical biosensor with lipase immobilized novel conductive polymer film has been developed for estimating triglyceride content in a variety of products. The portable biosensor can bring down the detection costs dramatically and can be used for varied purposes. It is based on cyclic voltammetry and has a three-electrode configuration system. Glassy carbon electrode is functionalized with nanoparticles embedded in polyethyleneimine and lipase is immobilized using glutaraldehyde. The strategy increases the electrochemical conductance manifold and overcomes the hindrance to lipase posed by membranes as it is oriented on the outside of the membrane. Thus, it increases the sensitivity and selectivity of detection. Results of scanning electron microscopy and FT-IR spectroscopy were used for characterizing the electrode surface. Linear range of the electrode for triglycerides is 100-500 mg/dL. The sensor was used successfully to determine triglyceride content in several real samples and the average recovery values lie from 95.47 % to 101.05 %.
血液中高水平的甘油三酯会使动脉变硬并堵塞，增加患心脏病和中风的风险。甘油三酯是各种食物中使用的油脂的重要成分。商业油制剂中的甘油三酯含量采用传统方法估算。在本研究中，已开发出一种带有固定化脂肪酶的新型导电聚合物膜的电化学生物传感器，用于估算各种产品中的甘油三酯含量。这种便携式生物传感器可大幅降低检测成本，并可用于多种目的。它基于循环伏安法，具有三电极配置系统。玻碳电极用嵌入聚乙烯亚胺的纳米颗粒进行功能化处理，并使用戊二醛固定脂肪酶。该策略增加了电化学电导率，并克服了膜对脂肪酶的阻碍，因为脂肪酶位于膜的外侧。因此，它提高了检测的灵敏度和选择性。扫描电子显微镜和傅里叶变换红外光谱的结果用于表征电极表面。该电极对甘油三酯的线性范围为100 - 500 mg/dL。该传感器已成功用于测定多个实际样品中的甘油三酯含量，平均回收率在95.47%至101.05%之间。
