Cotechini Tiziana, Jones Oliver, Hindmarch Charles Colin Thomas
Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
Queen's Cardiopulmonary Unit (QCPU), Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Kingston, ON, Canada.
Methods Mol Biol. 2023;2614:1-15. doi: 10.1007/978-1-0716-2914-7_1.
In situ profiling of the tumor-immune microenvironment (TiME) requires the ability to co-localize and detect multiple proteins simultaneously. Imaging mass cytometry (IMC), using the Hyperion™ imaging system is a novel multiplex imaging modality that currently enables detection of up to 50 markers on fixed tissues at subcellular resolution and thus has the potential to inform both pre-clinical and clinical research by providing investigators with spatially resolved information about the TiME. Here we provide an overview of the IMC workflow from sample fixation to analysis, with a focus on multiplex panel design and tissue staining.
肿瘤免疫微环境（TiME）的原位分析需要同时共定位和检测多种蛋白质的能力。使用Hyperion™成像系统的成像质谱流式细胞术（IMC）是一种新型的多重成像技术，目前能够在亚细胞分辨率下检测固定组织上多达50种标志物，因此有潜力通过为研究人员提供有关TiME的空间分辨信息，为临床前和临床研究提供参考。在此，我们概述了从样本固定到分析的IMC工作流程，重点是多重检测板设计和组织染色。
