Babes Liane, Yipp Bryan George, Senger Donna Lorraine
Lady Davis Institute for Medical Research, Montreal, QC, Canada.
Calvin, Phoebe and Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2023;2614:383-396. doi: 10.1007/978-1-0716-2914-7_23.
Real-time in vivo imaging has become an integral tool for the investigation and understanding of cellular processes in health and disease at single-cell resolution. This includes the dynamic and complex cellular interactions that occur during cancer progression and the subsequent metastatic dissemination of tumor cells to sites distant from the primary tumor. Herein we outline the methodology for the establishment and intravital imaging of the pulmonary metastatic niche, a preferred site of metastasis for many cancers, and describe the implementation of a lung window to visualize and dissect the intricate behaviour of multiple cell types within this environment. We also address the advantages and limitations of this high-resolution technology.
实时体内成像已成为以单细胞分辨率研究和理解健康与疾病中细胞过程的不可或缺的工具。这包括癌症进展过程中发生的动态且复杂的细胞相互作用，以及随后肿瘤细胞向远离原发肿瘤部位的转移扩散。在此，我们概述了肺转移微环境（许多癌症的首选转移部位）的建立和活体成像方法，并描述了肺窗的应用，以可视化和剖析该环境中多种细胞类型的复杂行为。我们还讨论了这项高分辨率技术的优缺点。
