Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA, 02114, USA.
Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA.
Adv Sci (Weinh). 2023 Dec;10(34):e2304886. doi: 10.1002/advs.202304886. Epub 2023 Oct 23.
Intravital microscopy (IVM) allows spatial and temporal imaging of different cell types in intact live tissue microenvironments. IVM has played a critical role in understanding cancer biology, invasion, metastases, and drug development. One considerable impediment to the field is the inability to interrogate the tumor microenvironment and its communication cascades during disease progression and therapeutic interventions. Here, a new implantable perfusion window chamber (PWC) is described that allows high-fidelity in vivo microscopy, local administration of stains and drugs, and longitudinal sampling of tumor interstitial fluid. This study shows that the new PWC design allows cyclic multiplexed imaging in vivo, imaging of drug action, and sampling of tumor-shed materials. The PWC will be broadly useful as a novel perturbable in vivo system for deciphering biology in complex microenvironments.
活体显微镜技术（IVM）可在完整的活体组织微环境中对不同类型的细胞进行时空成像。IVM 在理解癌症生物学、入侵、转移和药物开发方面发挥了关键作用。该领域的一个相当大的障碍是无法在疾病进展和治疗干预期间探究肿瘤微环境及其通讯级联。这里，描述了一种新的可植入式灌注窗室（PWC），它允许进行高保真度的体内显微镜检查、局部施用染色剂和药物以及肿瘤间质液的纵向采样。这项研究表明，新的 PWC 设计允许在体内进行循环复用成像、药物作用成像和肿瘤脱落物质的采样。PWC 将作为一种新型的可扰动活体系统，广泛用于破译复杂微环境中的生物学。
