Beijing Engineering Research Center of Environmental Material for Water Purification, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Beijing Engineering Research Center of Environmental Material for Water Purification, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Bioresour Technol. 2023 Feb;370:128557. doi: 10.1016/j.biortech.2022.128557. Epub 2022 Dec 29.
Understanding the relationship between dynamic microbial networks and functional stability is critical for the stable operation of anammox systems. Here, by operating an anammox reactor under constant condition over 250 days, it was found that the relative abundance of Planctomycetota gradually decreased while Chloroflexi and Proteobacteria increased, with stochasticity predominating the bacterial assembly as the reactor operation. Network analysis revealed a successional dynamic pattern of microbial interaction despite stable performance. The variation of subnetworks indicated Chloroflexi and Proteobacteria alternately played important role in anammox microbial network, and the negative relationship between anammox bacteria and heterotrophs could achieve a balance to keep functional stability under long-term operation. Furthermore, the identified keystone species mainly belonged to heterotrophs that were critical in maintaining network structure and system function. The results of this study revealed clear changing patterns of microbial community and network succession, which could provide valuable reference for other stably operated bioreactors.
理解动态微生物网络与功能稳定性之间的关系对 anammox 系统的稳定运行至关重要。在这里，通过在 250 天的时间内使 anammox 反应器在恒条件下运行，发现 Planctomycetota 的相对丰度逐渐降低，而 Chloroflexi 和 Proteobacteria 增加，随着反应器运行，随机性在细菌组装中占主导地位。网络分析揭示了尽管性能稳定，但微生物相互作用的演替动态模式。亚网络的变化表明 Chloroflexi 和 Proteobacteria 交替在 anammox 微生物网络中发挥重要作用，anammox 细菌与异养菌之间的负相关关系可以在长期运行中达到功能稳定的平衡。此外，鉴定的关键种主要属于在维持网络结构和系统功能方面至关重要的异养菌。本研究的结果揭示了微生物群落和网络演替的明显变化模式，可为其他稳定运行的生物反应器提供有价值的参考。
