Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold.

BACKGROUND

Methane (CH) and brassinosteroids (BRs) are important signaling molecules involved in a variety of biological processes in plants.

RESULTS

Here, marigold (Tagetes erecta L. 'Marvel') was used to investigate the role and relationship between CH and BRs during adventitious root (AR) formation. The results showed a dose-dependent effect of CH and BRs on rooting, with the greatest biological effects of methane-rich water (MRW, CH donor) and 2,4-epibrassinolide (EBL) at 20% and 1 μmol L, respectively. The positive effect of MRW on AR formation was blocked by brassinoazole (Brz, a synthetic inhibitor of EBL), indicating that BRs might be involved in MRW-regulated AR formation. MRW promoted EBL accumulation during rooting by up-regulating the content of campestanol (CN), cathasterone (CT), and castasterone (CS) and the activity of Steroid 5α-reductase (DET2), 22α-hydroxylase (DWF4), and BR-6-oxidase (BR6ox), indicating that CH could induce endogenous brassinolide (BR) production during rooting. Further results showed that MRW and EBL significantly down-regulated the content of cellulose, hemicellulose and lignin during rooting and significantly up-regulated the hydrolase activity, i.e. cmcase, xylanase and laccase. In addition, MRW and EBL also significantly promoted the activity of two major cell wall relaxing factors, xyloglucan endotransglucosylase/hydrolase (XTH) and peroxidase, which in turn promoted AR formation. While, Brz inhibited the role of MRW on these substances.

CONCLUSIONS

BR might be involved in CH-promoted AR formation by increasing cell wall relaxation.