Laboratory of proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Vet Q. 2023 Dec;43(1):1-13. doi: 10.1080/01652176.2023.2164908.
Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production.
To investigate the difference in the serum proteome between the cows with RP and the physiologic puerperium (PP).
MATERIAL & METHODS: Analysis of serum samples from nine cows with RP and six with PP using high-resolution liquid chromatography-tandem mass spectrometry approach. The proteins differing in the relative abundance between the PP and RP groups were classified using the Protein Analysis Through Evolutionary Relationship tool. For the pathway enrichment analysis, the REACTOME tool, with the human genome as the background, was employed. The criterion for significance was the false discovery rate corrected P-value less than 0.05.
In total 651 proteins were identified with altered relative abundance of ten proteins. Among them, seven had higher, and three showed lower relative abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in lipoprotein metabolism, and the remaining ones associated with for instance redox homeostasis, post-translational modification, and scavenging. Finally, the validation of the proteomic results showed that haptoglobin and lipopolysaccharide-binding protein levels reliably differentiated between the RP and PP groups.
The pattern of serum proteome alterations in the cows with RP mirrored several interplaying mechanisms underlying the systematic response to the presence of RP, therefore representing a source to mine for predictive or prognostic biomarkers.
奶牛产后胎衣不下（RP）是一种较为常见的疾病，对其健康状况和产奶量有很大的负面影响。
研究胎衣不下奶牛与生理产褥期奶牛血清蛋白质组的差异。
采用高分辨率液相色谱-串联质谱技术对 9 头 RP 奶牛和 6 头生理产褥期奶牛的血清样本进行分析。使用 Protein Analysis Through Evolutionary Relationship 工具对差异表达蛋白进行分类。使用 REACTOME 工具，以人类基因组为背景，对通路进行富集分析。差异蛋白的筛选标准为校正后的错误发现率（FDR）小于 0.05。
共鉴定出 651 种相对丰度改变的蛋白，其中 10 种蛋白的相对丰度差异显著。与生理产褥期相比，RP 奶牛血清中 7 种蛋白的相对丰度升高，3 种蛋白的相对丰度降低。差异表达蛋白参与了 15 条通路：其中 6 条与止血有关，3 条与脂蛋白代谢有关，其余的则与氧化还原平衡、翻译后修饰和清除等有关。最后，对蛋白质组学结果进行验证，发现触珠蛋白和脂多糖结合蛋白水平可可靠地区分 RP 组和生理产褥期组。
RP 奶牛血清蛋白质组的变化模式反映了系统对 RP 存在的多种相互作用的机制，因此可以作为挖掘预测或预后生物标志物的来源。
