1Department of Agricultural, Food and Nutritional Science,University of Alberta,Edmonton,AB,Canada T6G 2P5.
2Departments of Biological Sciences and Computing Science,University of Alberta,Edmonton,AB,Canada T6G 2E9.
Animal. 2018 May;12(5):1050-1059. doi: 10.1017/S1751731117002506. Epub 2017 Oct 16.
A targeted quantitative metabolomics approach was used to study temporal changes of serum metabolites in cows that normally released their fetal membranes and those that retained the placenta. We identified and measured serum concentrations of 128 metabolites including amino acids, acylcarnitines, biogenic amines, glycerophospholipids, sphingolipids and hexose at -8 and -4 weeks before parturition, during the week of retained placenta (RP) diagnosis, and at +4 and +8 weeks after parturition. In addition, we aimed at identifying metabolite signatures of pre-RP in the serum that might be used as predictive biomarkers for risk of developing RP in dairy cows. Results revealed major alterations in the metabolite fingerprints of pre-RP cows starting as early as -8 weeks before parturition and continuing as far as +8 weeks after calving. Biomarker candidates found in this study are mainly biomarkers of inflammation which might not be specific to RP. Therefore, the relevance of serum Lys, Orn, acetylornithine, lysophophatidylcholine LysoPC a C28:0, Asp, Leu and Ile as potential serum biomarkers for prediction of risk of RP in dairy cows will have to be tested in the future. In addition, lower concentrations of LysoPCs, Trp, and higher kynurenine in the serum during prepartum and the week of occurrence of RP suggest involvement of inflammation in the pathobiology of RP.
采用靶向定量代谢组学方法研究了正常分娩和胎盘滞留奶牛分娩前-8 周和-4 周、胎盘滞留诊断周和分娩后+4 周和+8 周血清代谢物的时间变化。我们鉴定并测量了 128 种代谢物的血清浓度，包括氨基酸、酰基辅酶 A、生物胺、甘油磷脂、神经鞘脂和己糖。此外，我们旨在确定血清中预 RP 的代谢物特征，这些特征可能作为预测奶牛发生 RP 风险的生物标志物。结果表明，早在分娩前-8 周，预 RP 奶牛的代谢指纹图谱就发生了重大变化，并持续到分娩后+8 周。本研究中发现的生物标志物候选物主要是炎症的生物标志物，可能不是 RP 特有的。因此，血清 Lys、Orn、acetylornithine、lysophosphatidylcholine LysoPC a C28:0、Asp、Leu 和 Ile 作为预测奶牛 RP 风险的潜在血清生物标志物的相关性有待进一步研究。此外，在围产期和 RP 发生周期间，血清中 LysoPCs、Trp 和较高的犬尿氨酸浓度降低，提示炎症参与了 RP 的病理生理学。
