Center for Studies in Constitution Research of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Biomed Res Int. 2022 Dec 23;2022:6464431. doi: 10.1155/2022/6464431. eCollection 2022.
Phlegm-dampness constitution as one of nine constitutions in traditional Chinese medicine (TCM) has been a high risk factor for glucolipid metabolic disorders (GLMD). Based on our previous findings, Hua Tan Qu Shi recipe (HTQSR) could effectively improve metabolic indicators of GLMD by targeting on phlegm-dampness constitution. However, the proteomic mechanisms of GLMD with the treatment of HTQSR targeting on phlegm-dampness constitution remain unknown.
Clinical participants from phlegm-dampness constitution with the prediabetic state (T), phlegm-dampness constitution with marginally elevated blood lipids (Z), and phlegm-dampness constitution before sickness (W) were included in this study, who orally took HTQSR for 12 weeks and, respectively, marked AT, AZ, and AW. Data-independent acquisition (DIA) and parallel reaction monitoring (PRM) were performed to identify the differential proteins; then, Venn analysis was used to investigate coexpressed and coregulated proteins. In addition, ingenuity pathway analysis (IPA) software was utilized to explore the related pathways and diseases and biofunctions.
LXR/RXR activation, acute phase response signaling, and production of nitric oxide and reactive oxygen species in macrophages were obviously activated between the T and AT groups, as well as the Z and AZ groups. In contrast, these three pathways were inhibited between the W and AW groups. Importantly, one coexpressed and coregulated differential protein, B2MG, was validated by PRM among all groups.
This work firstly reported the underlying proteomic mechanisms of GLMD with the treatment of HTQSR targeting on phlegm-dampness constitution, indicating that intervention of phlegm-dampness constitution might be a novel strategy for the preventive treatment of GLMD.
痰湿体质作为中医九种体质之一，是糖脂代谢紊乱（GLMD）的高风险因素。基于我们之前的研究发现，化痰祛湿方（HTQSR）可通过针对痰湿体质改善 GLMD 的代谢指标。然而，针对痰湿体质的 GLMD 治疗靶点 HTQSR 的蛋白质组学机制尚不清楚。
本研究纳入了具有糖尿病前期状态的痰湿体质患者（T 组）、血脂偏高的痰湿体质患者（Z 组）和患病前的痰湿体质患者（W 组），这些患者均口服 HTQSR 治疗 12 周，分别标记为 AT、AZ 和 AW。采用数据非依赖性采集（DIA）和平行反应监测（PRM）技术来鉴定差异蛋白；然后，利用 Venn 分析来研究共表达和核心调控蛋白。此外，还使用了 IPA 软件来探讨相关通路和疾病及生物功能。
LXR/RXR 激活、急性期反应信号和巨噬细胞中一氧化氮和活性氧的产生在 T 组和 AT 组之间以及 Z 组和 AZ 组之间明显被激活。相比之下，在 W 组和 AW 组之间，这三个通路被抑制。重要的是，在所有组中，B2MG 这一共表达和核心调控差异蛋白通过 PRM 得到了验证。
本研究首次报道了针对痰湿体质的 HTQSR 治疗 GLMD 的潜在蛋白质组学机制，表明干预痰湿体质可能是 GLMD 预防治疗的一种新策略。
