Mello Ericson R, Oliveira Mariana S, Andrade Lucas de Assis S, Cardozo Sergian V, Oliveira Águida A, Lima Viviane M, Berto Bruno P
Programa de Pós-graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
Programa de Pós-graduação em Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
Curr Res Parasitol Vector Borne Dis. 2022 Jan 31;2:100079. doi: 10.1016/j.crpvbd.2022.100079. eCollection 2022.
Mello & Berto n. sp. is described based on material from the golden-crowned warbler (Deppe) captured in the Itatiaia National Park (Parque Nacional do Itatiaia), a conservation unit in south-eastern Brazil. Oöcysts of the new species are ellipsoidal to ovoidal, measuring on average 25.2 × 21.1 μm, with a smooth, bi-layered wall, 1.6 μm thick. Micropyle and oöcyst residuum are both absent, but one to three polar granules are present. Sporocysts are ellipsoidal to lemon-shaped, measuring on average 15.3 × 9.5 μm, with a knob-like Stieda body and a trapezoidal sub-Stieda body. Sporocyst residuum is present, usually as a body of membrane-bound granules. Sporozoites are vermiform, with refractile bodies. Four of the 19 warblers captured (21%) were infected with the new species. Molecular analysis of the mitochondrial cytochrome oxidase subunit 1 (1) gene revealed a similarity of 99.5% between the new species and Yang, Brice, Elliot & Ryan, 2015 from island canaries (L.) in Western Australia. The oöcysts of n. sp. can be distinguished from the four other spp. recorded in hosts of the Parulidae, and from the molecularly most closely related species, by the typical ellipsoidal to lemon-shaped sporocysts, with small sub-Stieda body and a membrane-bound sporocyst residuum. Therefore, based on the morphological and molecular features, n. sp. is the fifth species described in a host of the family Parulidae and the first molecularly characterized sequencing the 1 gene.
梅洛氏和贝托氏新种是根据在巴西东南部的一个保护单位伊塔蒂亚伊国家公园（伊塔蒂亚伊国家公园）捕获的金冠莺（德普）的材料描述的。新种的卵囊呈椭圆形至卵形，平均大小为25.2×21.1μm，壁光滑，双层，厚1.6μm。微孔和卵囊残余物均不存在，但有一至三个极粒。孢子囊呈椭圆形至柠檬形，平均大小为15.3×9.5μm，有一个瘤状的斯氏体和一个梯形的亚斯氏体。孢子囊残余物存在，通常为膜结合颗粒体。子孢子呈蠕虫状，有折光体。捕获的19只莺中有4只（21%）感染了新种。线粒体细胞色素氧化酶亚基1（1）基因的分子分析显示，新种与2015年来自西澳大利亚岛金丝雀（L.）的杨、布赖斯、埃利奥特和瑞安种之间的相似度为99.5%。新种的卵囊可通过典型的椭圆形至柠檬形孢子囊、小的亚斯氏体和膜结合的孢子囊残余物，与在森莺科宿主中记录的其他四个种以及分子上最密切相关的物种区分开来。因此，根据形态和分子特征，新种是森莺科宿主中描述的第五个种，也是第一个通过对1基因进行测序进行分子特征鉴定的种。
