Frank Dmitry, Gruenbaum Benjamin F, Semyonov Michael, Binyamin Yair, Severynovska Olena, Gal Ron, Frenkel Amit, Knazer Boris, Boyko Matthew, Zlotnik Alexander
Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev;
Department of Anesthesiology and Perioperative Medicine, Mayo Clinic.
J Vis Exp. 2022 Dec 16(190). doi: 10.3791/64548.
Competition over resources such as food, territory, and mates significantly influences relationships within animal species and is mediated through social hierarchies that are often based on dominant-submissive relationships. The dominant-submissive relationship is a normal behavioral pattern among the individuals of a species. Traumatic brain injury is a frequent cause of social interaction impairment and the reorganization of dominant-submissive relationships in animal pairs. This protocol describes submissive behavior in adult male Sprague-Dawley rats after the induction of traumatic brain injury using a fluid-percussion model compared to naive rats through a series of dominant-submissive tests performed between 29 days and 33 days after induction. The dominant-submissive behavior test shows how brain injury can induce submissive behavior in animals competing for food. After traumatic brain injury, the rodents were more submissive, as indicated by them spending less time at the feeder and being less likely to arrive first at the trough compared to the control animals. According to this protocol, submissive behavior develops after traumatic brain injury in adult male rats.
对食物、领地和配偶等资源的竞争显著影响动物物种内部的关系，并通过通常基于主从关系的社会等级制度来调节。主从关系是一个物种个体之间的正常行为模式。创伤性脑损伤是动物对中社会互动受损和主从关系重新组织的常见原因。本方案描述了使用液压冲击模型诱导创伤性脑损伤后成年雄性Sprague-Dawley大鼠的顺从行为，与未受伤大鼠相比，通过在诱导后29天至33天之间进行的一系列主从测试。主从行为测试显示了脑损伤如何在争夺食物的动物中诱发顺从行为。创伤性脑损伤后，啮齿动物表现得更加顺从，与对照动物相比，它们在喂食器处停留的时间更短，不太可能最先到达食槽。根据本方案，成年雄性大鼠创伤性脑损伤后会出现顺从行为。
