Abiopep S.L., Parque Científico de Murcia, Ctra. de Madrid, Km 388, Complejo de Espinardo, 30100, Espinardo, Murcia, Spain.
Department of Stress Biology and Plant Pathology, Centro de Edafología y Biología Aplicada del Segura (CEBAS)-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain.
Arch Virol. 2023 Jan 3;168(1):16. doi: 10.1007/s00705-022-05669-3.
We determined the complete genome sequence of a new virus infecting Ecballium elaterium ('cohombrillo amargo') plants, a weed species common on the borders of cultivated fields in the Mediterranean region. The genome of this virus is composed of two molecules of monocistronic positive-sense RNA, 6,934 and 3,501 nucleotides in length, excluding their poly(A) tails. The highest amino acid sequence similarity (50 % identity) in the Pro-Pol core region encoded by RNA 1 was observed in the corresponding protein of strawberry latent ringspot virus. Based on pairwise comparisons and phylogenetic analysis, this virus, tentatively named "cohombrillo-associated virus" (CoAV), appears to be a member of a new species in the genus Stralarivirus (family Secoviridae), for which the name "Stralarivirus elaterii" is proposed. This new virus has different putative cleavage patterns from members of other species belonging to this genus.
我们确定了一种感染 Ecballium elaterium（“苦瓠瓜”）植物的新型病毒的完整基因组序列，这种杂草物种在地中海地区的农田边缘很常见。该病毒的基因组由两条单顺反子正链 RNA 分子组成，长度分别为 6934 个和 3501 个核苷酸，不包括它们的 poly(A)尾巴。RNA1 编码的 Pro-Pol 核心区域中的最高氨基酸序列相似性（50%同一性）在草莓潜隐环斑病毒的相应蛋白中观察到。基于成对比较和系统发育分析，这种病毒暂命名为“苦瓠associated 病毒”（CoAV），似乎是 Stalarivirus 属（肠病毒科）的一个新种成员，建议将其命名为“Stalarivirus elaterii”。与属于该属的其他种成员相比，这种新病毒具有不同的假定切割模式。
