Igori Davaajargal, Shin Ah-Young, Hwang Un Sun, Choi Eung Kyoo, Kwon Suk-Yoon, Moon Jae Sun
Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia.
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Arch Virol. 2023 Feb 1;168(2):77. doi: 10.1007/s00705-023-05699-5.
The complete nucleotide sequence of a novel gondre (Cirsium setidens)-infecting virus, provisionally named "cirsium virus A" (CiVA), was determined by high-throughput and Sanger sequencing, revealing a genome organization typical of fabaviruses. RNA1 and RNA2 are 5,828 and 3,478 nucleotides long, excluding the 3'-terminal poly(A) tails, each containing a single open reading frame. The highest sequence identity values for the CiVA coat protein and proteinase-polymerase, compared with known fabavirus sequences, were 59.09% and 69.68%, respectively, falling below the current thresholds for Fabavirus species demarcation. Our findings support classifying CiVA as a novel putative member of the genus Fabavirus, subfamily Comovirinae, family Secoviridae.
通过高通量测序和桑格测序确定了一种新型感染贡德尔蓟（Cirsium setidens）的病毒的完整核苷酸序列，该病毒暂命名为“蓟病毒A”（CiVA），其基因组结构为蚕豆病毒属所特有。RNA1和RNA2长度分别为5828和3478个核苷酸，不包括3'末端的聚腺苷酸尾，每个都含有一个单一的开放阅读框。与已知蚕豆病毒序列相比，CiVA外壳蛋白和蛋白酶-聚合酶的最高序列同一性值分别为59.09%和69.68%，低于当前蚕豆病毒属物种划分的阈值。我们的研究结果支持将CiVA归类为蚕豆病毒属、豇豆花叶病毒亚科、番茄病毒科的一个新的推定成员。
