Bionanomechanics Lab , Instituto de Micro y Nanotecnología, IMN-CNM (CSIC) , Isaac Newton 8 (PTM) , E-28760 Tres Cantos , Madrid , Spain.
ACS Sens. 2019 Dec 27;4(12):3325-3332. doi: 10.1021/acssensors.9b02038. Epub 2019 Dec 12.
The study of biophysical properties of single cells is becoming increasingly relevant in cell biology and pathology. The measurement and tracking of magnitudes such as cell stiffness, morphology, and mass or refractive index have brought otherwise inaccessible knowledge about cell physiology, as well as innovative methods for high-throughput label-free cell classification. In this work, we present hollow resonator devices based on suspended glass microcapillaries for the simultaneous measurement of single-cell buoyant mass and reflectivity with a throughput of 300 cells/minute. In the experimental methodology presented here, both magnitudes are extracted from the devices' response to a single probe, a focused laser beam that enables simultaneous readout of changes in resonance frequency and reflected optical power of the devices as cells flow within them. Through its application to MCF-7 human breast adenocarcinoma cells and MCF-10A nontumorigenic cells, we demonstrate that this mechano-optical technique can successfully discriminate pathological from healthy cells of the same tissue type.
单细胞的生物物理特性研究在细胞生物学和病理学领域变得越来越重要。对细胞硬度、形态、质量或折射率等参数的测量和跟踪，为我们提供了关于细胞生理学的原本难以获得的知识，以及用于高通量无标记细胞分类的创新方法。在这项工作中，我们提出了基于悬空玻璃微管的空心谐振器器件，用于以 300 个细胞/分钟的通量同时测量单细胞的浮力质量和反射率。在本文提出的实验方法中，这两个参数都是从器件对单个探针的响应中提取的，该探针是一个聚焦激光束，当细胞在其中流动时，它可以同时读取器件的共振频率变化和反射光功率。通过将其应用于 MCF-7 人乳腺癌腺癌细胞和 MCF-10A 非肿瘤细胞，我们证明这种机械光学技术可以成功地区分同组织类型的病理细胞和健康细胞。
