Department of Integrative Physiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Cardiovascular Research Institute, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Mamm Genome. 2023 Jun;34(2):156-165. doi: 10.1007/s00335-022-09976-7. Epub 2023 Jan 3.
Comprehensive detailed characterization of new mouse models can be challenging due to the individual focus involved in developing these models. Often models are engineered to test a specific hypothesis in a limited number of tissues, stages, and/or other contexts. Whether or not the model produces the desired phenotypes, phenotyping beyond the desired context can be extremely work intensive and these studies are often not undertaken. However, the general information resulting from broader phenotyping can be invaluable to the wider scientific community. The International Mouse Phenotyping Consortium (IMPC) and its subsidiaries, like the Knockout Mouse Project (KOMP), has made great strides in streamlining this process. In particular, the use of microCT has been an invaluable resource in examining internal organ systems throughout fetal/developmental stages. Here, we provide several novel vignettes demonstrating the utility of microCT in uncovering cardiac phenotypes both based on human disease correlations and those that are unpredicted.
由于开发这些模型涉及到个别关注点，因此全面详细地描述新的小鼠模型可能具有挑战性。通常，这些模型是为了在有限的组织、阶段和/或其他背景下测试特定假设而设计的。无论模型是否产生预期的表型，超出预期背景的表型分析都可能非常耗费精力，而且这些研究通常没有进行。然而，更广泛的表型分析所产生的一般信息对更广泛的科学界可能是非常有价值的。国际小鼠表型分析联盟（IMPC）及其子公司，如敲除小鼠项目（KOMP），在简化这一过程方面取得了重大进展。特别是，微计算机断层扫描（microCT）的使用在检查整个胎儿/发育阶段的内部器官系统方面是非常有价值的资源。在这里，我们提供了几个新的案例研究，展示了 microCT 在揭示基于人类疾病相关性和未预测的心脏表型方面的应用。
