Department of Stomatology, Shijiazhuang Xingye Shengrui Stomatological Hospital, Shijlazhuang, Hebei Province, PR China.
Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijlazhuang,Hebei Province, PR China.
Medicine (Baltimore). 2022 Dec 30;101(52):e32595. doi: 10.1097/MD.0000000000032595.
Oral squamous cell carcinoma is a malignant tumor that occurs in the oral cavity, with poor prognosis and easy recurrence. However, the relationship between MKI67 and oral squamous cell carcinoma remains unclear. The oral squamous cell carcinoma datasets GSE138206, GSE146483 and GSE184616 were downloaded from the gene expression omnibus database, and the differentially expressed genes (DEGs) were screened. The protein-protein interaction network was constructed and analyzed by search tool for the retrieval of interacting genes database and Cytoscape software. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) were used for functional enrichment analysis. GO and KEGG analyses were performed on the whole genome, as formulated by gene set enrichment analysis. comparative toxicogenomics database was used to identify the diseases most associated with the core genes. TargetScan was used to screen miRNA regulating central DEGs. A total of 1472 DEGs were identified. GO analysis showed that the differentially expressed genes were mainly enriched in the tissues of extracellular matrix, type i interferon signaling pathway, human papillomavirus infection, adhesion spot, hepatitis C and ECM-receptor interaction. Enrichment items were similar to GO and KEGG enrichment items of differentially expressed genes. 10 core genes were obtained, and their expression was different between oral squamous cell carcinoma and normal tissue samples. MKI67 is highly expressed in oral squamous cell carcinoma and may be an oncogene in oral squamous cell carcinoma.
口腔鳞状细胞癌是一种发生在口腔的恶性肿瘤，预后较差，容易复发。然而，MKI67 与口腔鳞状细胞癌之间的关系尚不清楚。从基因表达综合数据库中下载了口腔鳞状细胞癌数据集 GSE138206、GSE146483 和 GSE184616，筛选差异表达基因（DEGs）。通过搜索工具检索基因相互作用数据库和 Cytoscape 软件构建和分析蛋白质-蛋白质相互作用网络。使用基因集富集分析对整个基因组进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）功能富集分析。比较毒理学基因组数据库用于识别与核心基因最相关的疾病。TargetScan 用于筛选调节中央 DEGs 的 miRNA。共鉴定出 1472 个 DEGs。GO 分析表明，差异表达基因主要富集在细胞外基质组织、I 型干扰素信号通路、人乳头瘤病毒感染、黏附斑、丙型肝炎和 ECM-受体相互作用中。富集项目与差异表达基因的 GO 和 KEGG 富集项目相似。获得了 10 个核心基因，它们在口腔鳞状细胞癌和正常组织样本中的表达不同。MKI67 在口腔鳞状细胞癌中高表达，可能是口腔鳞状细胞癌的癌基因。
