Department of Stomatology, Tangshan Gongren Hospital, Tangshan City, China.
Department of Stomatology, The First Hospital of Hebei Medical University, Shijiazhuang City, China.
Medicine (Baltimore). 2024 Apr 19;103(16):e37831. doi: 10.1097/MD.0000000000037831.
Oral squamous cell carcinoma (OSCC) is a malignant tumor that occurs in oral cavity and is dominated by squamous cells. The relationship between CDK1, CCNA2, and OSCC is still unclear. The OSCC datasets GSE74530 and GSE85195 configuration files were downloaded from the Gene Expression Omnibus (GEO) database and were derived from platforms GPL570 and GPL6480. Differentially expressed genes (DEGs) were screened. The weighted gene co-expression network analysis, functional enrichment analysis, gene set enrichment analysis, construction and analysis of protein-protein interaction (PPI) network, Comparative Toxicogenomics Database analysis were performed. Gene expression heatmap was drawn. TargetScan was used to screen miRNAs that regulate central DEGs. A total of 1756 DEGs were identified. According to Gene Ontology (GO) analysis, they were predominantly enriched in processes related to organic acid catabolic metabolism, centromeric, and chromosomal region condensation, and oxidoreductase activity. In Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the DEGs were mainly concentrated in metabolic pathways, P53 signaling pathway, and PPAR signaling pathway. Weighted gene co-expression network analysis was performed with a soft-thresholding power set at 9, leading to the identification of 6 core genes (BUB1B, CCNB1, KIF20A, CCNA2, CDCA8, CDK1). The gene expression heatmap revealed that core genes (CDK1, CCNA2) were highly expressed in OSCC samples. Comparative Toxicogenomics Database analysis demonstrated associations between the 6 genes (BUB1B, CCNB1, KIF20A, CCNA2, CDCA8, CDK1) and oral tumors, precancerous lesions, inflammation, immune system disorders, and tongue tumors. The associated miRNAs for CDK1 gene were hsa-miR-203a-3p.2, while for CCNA2 gene, they were hsa-miR-6766-3p, hsa-miR-4782-3p, and hsa-miR-219a-5p. CDK1 and CCNA2 are highly expressed in OSCC. The higher the expression of CDK1 and CCNA2, the worse the prognosis.
口腔鳞状细胞癌（OSCC）是一种发生在口腔的恶性肿瘤，以鳞状细胞为主。CDK1、CCNA2 与 OSCC 的关系尚不清楚。从基因表达综合数据库（GEO）下载 OSCC 数据集 GSE74530 和 GSE85195 的配置文件，来源于 GPL570 和 GPL6480 平台。筛选差异表达基因（DEGs）。进行加权基因共表达网络分析、功能富集分析、基因集富集分析、构建和分析蛋白质-蛋白质相互作用（PPI）网络、比较毒理学基因组数据库分析。绘制基因表达热图。使用 TargetScan 筛选调节中心 DEGs 的 miRNA。共鉴定出 1756 个 DEGs。根据基因本体论（GO）分析，它们主要富集在与有机酸分解代谢、着丝粒和染色体区域浓缩以及氧化还原酶活性相关的过程中。在京都基因与基因组百科全书（KEGG）分析中，DEGs 主要集中在代谢途径、P53 信号通路和 PPAR 信号通路中。使用软阈值功率设置为 9 进行加权基因共表达网络分析，确定了 6 个核心基因（BUB1B、CCNB1、KIF20A、CCNA2、CDCA8、CDK1）。基因表达热图显示核心基因（CDK1、CCNA2）在 OSCC 样本中高表达。比较毒理学基因组数据库分析表明，6 个基因（BUB1B、CCNB1、KIF20A、CCNA2、CDCA8、CDK1）与口腔肿瘤、癌前病变、炎症、免疫系统紊乱和舌肿瘤有关。CDK1 基因的相关 miRNA 是 hsa-miR-203a-3p.2，而 CCNA2 基因的相关 miRNA 是 hsa-miR-6766-3p、hsa-miR-4782-3p 和 hsa-miR-219a-5p。CDK1 和 CCNA2 在 OSCC 中高表达。CDK1 和 CCNA2 的表达水平越高，预后越差。
