Xiao Liping, Li Xin, Wang Jing-Jing, Quan Xue-Min, Zhao Chang-Song
Cardiovascular Internal Medicine, The Second Hospital of Qinhuangdao, Changli County, Qinhuangdao, Hebei Province, China.
Department of Orthopaedics, Beijing Ditan Hospital Affiliated to Capital Medical University, Chaoyang District, Beijing, China.
Medicine (Baltimore). 2024 Nov 22;103(47):e40692. doi: 10.1097/MD.0000000000040692.
Acquired immunodeficiency syndrome is a systemic infectious disease caused by human immunodeficiency virus infection, which could attack the bones and heart. However, the relationship between Nuclear Complex Associated 3 Homolog (NOC3L) and DEAD box helicase 17 (DDX17) and acquired immunodeficiency complicated with viral myocarditis and osteoporosis is unclear. The acquired immune deficiency dataset GSE140713, GSE147162 and the osteoporosis dataset (GSE230665), and viral myocarditis dataset (GSE150392) configuration files were generated from gene expression omnibus. The differentially expressed genes (DEGs) were screened and performed weighted gene co-expression network analysis. Construction and analysis of protein-protein interaction network. Functional enrichment analysis, gene set enrichment analysis, immune infiltration analysis, gene expression heatmap, and comparative toxicogenomics database analysis were performed. TargetScan screens miRNAs of DEGs. Thousand three hundred thirty-five DEGs were identified. According to gene ontology, they are mainly concentrated in the regulation of RNA biosynthesis, cytoplasmic ribosome, and the DNA binding transcription factor activity. In Kyoto Encyclopedia of Genes and Genomes analysis, they are mainly concentrated in TGF-β signal pathway, Notch signaling pathway, cAMP signaling pathway, and Apelin signaling pathway. Gene set enrichment analysis shows that DEGs are mainly enriched in cytoplasmic ribosome, transcriptional regulator activity, DNA binding transcription factor activity, TGF-β signal pathway, and Notch signal pathway. In the enrichment project of Metascape, tyrosine kinase receptor signaling, growth regulation, and enzyme-linked receptor protein signaling pathways can be seen in the gene ontology enrichment project. Four core genes (NOC3L, WDR46, SDAD1, and DDX17) were obtained. Core genes (NOC3L, WDR46, SDAD1, and DDX17) were low expressed in both acquired immunodeficiency and osteoporosis samples. Comparative toxicogenomics database analysis showed that core genes (NOC3L, WDR46, SDAD1, and DDX17) were associated with inflammation necrosis. The expressions of NOC3L and DDX17 are low in acquired immunodeficiency combined with viral myocarditis and osteoporosis.
获得性免疫缺陷综合征是由人类免疫缺陷病毒感染引起的一种全身性传染病，可侵袭骨骼和心脏。然而，核复合体相关3同源物（NOC3L）与DEAD盒解旋酶17（DDX17）之间的关系以及获得性免疫缺陷合并病毒性心肌炎和骨质疏松症的情况尚不清楚。从基因表达综合数据库生成了获得性免疫缺陷数据集GSE140713、GSE147162以及骨质疏松症数据集（GSE230665）和病毒性心肌炎数据集（GSE150392）的配置文件。筛选差异表达基因（DEG）并进行加权基因共表达网络分析。构建并分析蛋白质-蛋白质相互作用网络。进行功能富集分析、基因集富集分析、免疫浸润分析、基因表达热图以及比较毒理基因组学数据库分析。TargetScan筛选DEG的微小RNA。共鉴定出1335个DEG。根据基因本体论，它们主要集中在RNA生物合成调控、细胞质核糖体以及DNA结合转录因子活性方面。在京都基因与基因组百科全书分析中，它们主要集中在转化生长因子-β信号通路、Notch信号通路、环磷酸腺苷信号通路和Apelin信号通路。基因集富集分析表明，DEG主要富集在细胞质核糖体、转录调节因子活性、DNA结合转录因子活性、转化生长因子-β信号通路和Notch信号通路。在Metascape的富集项目中，在基因本体论富集项目中可以看到酪氨酸激酶受体信号传导、生长调节和酶联受体蛋白信号通路。获得了四个核心基因（NOC3L、WDR46、SDAD1和DDX17）。核心基因（NOC3L、WDR46、SDAD1和DDX17）在获得性免疫缺陷和骨质疏松症样本中均低表达。比较毒理基因组学数据库分析表明，核心基因（NOC3L、WDR46、SDAD1和DDX17）与炎症坏死相关。NOC3L和DDX17在获得性免疫缺陷合并病毒性心肌炎和骨质疏松症中的表达较低。
