Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Ann Parasitol. 2022;68(3):563-568. doi: 10.17420/ap6803.463.
Anthelmintic resistance against gastrointestinal nematodes especially H. contortus of sheep and goat is a global issue. To address the gravity and extension of AR in Bangladesh, genotyping of 160 adult H. contortus parasitesw ere performed to confirm benzimidazole resistance allele from different geographic zones of Bangladesh based on allele specific PCR (AS-PCR). The genotype frequencies were 9.4% for homozygous resistant (rr), 61.2% for heterozygous (rS) and 29.4% for homozygous susceptible (SS) among the selected areas. The allelic frequency of the mutation conferring resistance (r) ranged from 27.5% to 52.5% indicating substantial existence of benzimidazole resistance in H. contortus in small ruminant nematodes. Therefore, it can be concluded that genotyping the F200Y polymorphism can be used to monitor the resistance and thereby to enhance the control on the development of anthelmintic resistance against H. contortus in small ruminant nematodes.
抗蠕虫药对绵羊和山羊胃肠道线虫（尤其是捻转血矛线虫）的耐药性是一个全球性问题。为了了解孟加拉国抗药性的严重程度和范围，我们对 160 个捻转血矛线虫成虫进行基因分型，基于等位基因特异性 PCR（AS-PCR），从孟加拉国不同地理区域确认苯并咪唑类耐药等位基因。在所选择的地区中，纯合耐药（rr）基因型的频率为 9.4%，杂合耐药（rS）基因型的频率为 61.2%，纯合敏感（SS）基因型的频率为 29.4%。赋予耐药性的突变等位基因（r）的等位基因频率为 27.5%至 52.5%，表明捻转血矛线虫中小反刍动物线虫中存在大量的苯并咪唑类耐药性。因此，可以得出结论，对 F200Y 多态性进行基因分型可以用于监测耐药性，从而加强对捻转血矛线虫的抗药性的控制。
