Qiao Baoxin, He Ying, Gao Xinglin, Liu Haiyan, Rao Gan, Su Qian, Ruan Zhiyan, Tang Zhaoxin, Hu Lianmei
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China; Guangxi Key Laboratory of Veterinary Biotechnology, Nanning, Guangxi, China; Key Laboratory of China(Guangxi)-ASEAN Cross-border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs of China, Nanning, China.
Food Chem Toxicol. 2023 Feb;172:113593. doi: 10.1016/j.fct.2022.113593. Epub 2022 Dec 31.
Aflatoxin B1 (AFB1), as the most toxic secondary metabolite produced by Aspergillus flavus, is a serious threat to human and animal health. Curcumin, a polyphenol from the plant turmeric, has demonstrated unique anti-damage properties in several studies. But, its ability to alleviate AFB1-induced liver damage in ducks and the underlying mechanisms are not completely elucidated. In this study, we investigated the intervention of curcumin on AFB1-induced hepatotoxicity in ducks. Research data showed that the combination of curcumin and AFB1 alleviated oxidative stress, reduced malondialdehyde (MDA) accumulation and relieved hepatotoxicity after 28 days of treatment, compared with AFB1. Also, curcumin upregulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream antioxidant enzymes (SOD, HO-1), which enhanced the antioxidant capacity of the liver. In addition, curcumin inhibited AFB1-induced lysosomal damage in the liver, with the character of reduced lysosomal membrane permeabilization, restored autophagic flux, and promoted lysosomal biogenesis, thereby enhancing the self-protective capacity of the liver. In conclusion, our results suggest that curcumin alleviates AFB1-induced duck hepatotoxicity by inhibiting oxidative stress and lysosomal damage.
黄曲霉毒素B1（AFB1）作为黄曲霉产生的毒性最强的次生代谢产物，对人类和动物健康构成严重威胁。姜黄素是一种从植物姜黄中提取的多酚，在多项研究中已显示出独特的抗损伤特性。但是，其减轻AFB1诱导的鸭肝脏损伤的能力及潜在机制尚未完全阐明。在本研究中，我们调查了姜黄素对AFB1诱导的鸭肝毒性的干预作用。研究数据表明，与AFB1组相比，姜黄素与AFB1联合处理28天后减轻了氧化应激，减少了丙二醛（MDA）积累并缓解了肝毒性。此外，姜黄素上调了核因子E2相关因子2（Nrf2）及其下游抗氧化酶（SOD、HO-1）的表达，增强了肝脏的抗氧化能力。另外，姜黄素抑制了AFB1诱导的肝脏溶酶体损伤，表现为溶酶体膜通透性降低、自噬流恢复以及溶酶体生物合成促进，从而增强了肝脏的自我保护能力。总之，我们的结果表明姜黄素通过抑制氧化应激和溶酶体损伤来减轻AFB1诱导的鸭肝毒性。
