Huang Li-Qiu, Ding Xin-Lei, Pan Xiao-Tong, Li Zhong-Qiu, Wang Kang, Xia Xing-Hua
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Chem Commun (Camb). 2023 Jan 19;59(7):876-879. doi: 10.1039/d2cc06110d.
Herein, a nanopipette-based thermocouple probe that possesses high temperature resolution, rapid response, good reversibility and stability was constructed and successfully applied for single-cell temperature sensing. Different intracellular temperatures were observed in diverse types of cells, which reveals differences in their metabolism levels. Temperature responses of cancer and normal cells against various exogenous drugs were also demonstrated. The spatially resolved temperature sensing of three-dimensional cell culture models unveils the existence of their inner temperature gradients. This work would facilitate drug screening and disease diagnosis.
在此，构建了一种基于纳米移液器的热电偶探针，该探针具有高温度分辨率、快速响应、良好的可逆性和稳定性，并成功应用于单细胞温度传感。在不同类型的细胞中观察到了不同的细胞内温度，这揭示了它们代谢水平的差异。还展示了癌细胞和正常细胞对各种外源药物的温度响应。三维细胞培养模型的空间分辨温度传感揭示了其内部温度梯度的存在。这项工作将有助于药物筛选和疾病诊断。
