Zhu Hanliang, Xu Haotian, Zhang Yue, Brodský Jan, Gablech Imrich, Korabečná Marie, Neuzil Pavel
School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, P. R. China.
Department of Microelectronics, The Faculty of Electrical Engineering and Communication Technology, Brno University of Technology, Technická 3058/10, Brno, 616 00, Czech Republic.
Adv Sci (Weinh). 2025 Jan;12(1):e2402135. doi: 10.1002/advs.202402135. Epub 2024 Oct 28.
The precise measurement of cell temperature and an in-depth understanding of thermogenic processes are critical in unraveling the complexities of cellular metabolism and its implications for health and disease. This review focuses on the mechanisms of local temperature generation within cells and the array of methods developed for accurate temperature assessment. The contact and noncontact techniques are introduced, including infrared thermography, fluorescence thermometry, and other innovative approaches to localized temperature measurement. The role of thermogenesis in cellular metabolism, highlighting the integral function of temperature regulation in cellular processes, environmental adaptation, and the implications of thermogenic dysregulation in diseases such as metabolic disorders and cancer are further discussed. The challenges and limitations in this field are critically analyzed while technological advancements and future directions are proposed to overcome these barriers. This review aims to provide a consolidated resource for current methodologies, stimulate discussion on the limitations and challenges, and inspire future innovations in the study of cellular thermodynamics.
精确测量细胞温度并深入了解产热过程对于揭示细胞代谢的复杂性及其对健康和疾病的影响至关重要。本综述聚焦于细胞内局部温度产生的机制以及为精确温度评估而开发的一系列方法。介绍了接触式和非接触式技术，包括红外热成像、荧光测温法以及其他用于局部温度测量的创新方法。进一步讨论了产热在细胞代谢中的作用，强调了温度调节在细胞过程、环境适应中的不可或缺的功能，以及产热失调在代谢紊乱和癌症等疾病中的影响。批判性地分析了该领域的挑战和局限性，同时提出了技术进步和未来方向以克服这些障碍。本综述旨在为当前方法提供一个综合资源，激发对局限性和挑战的讨论，并激发细胞热力学研究的未来创新。
