Nie Yuanyuan, Li Yang, Liu Menghui, Ma Binran, Sui Xinying, Chen Jiacai, Yu Yanchong, Dong Chun-Hai
College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
Plant Cell Rep. 2023 Mar;42(3):549-559. doi: 10.1007/s00299-022-02976-6. Epub 2023 Jan 4.
Arabidopsis nucleoporin involved in the regulation of ethylene signaling via controlling of nucleocytoplasmic transport of mRNAs. The two-way transport of mRNAs between the nucleus and cytoplasm are controlled by the nuclear pore complex (NPC). In higher plants, the NPC contains at least 30 nucleoporins. The Arabidopsis nucleoporins are involved in various biological processes such as pathogen interaction, nodulation, cold response, flowering, and hormone signaling. However, little is known about the regulatory functions of the nucleoporin NUP160 and NUP96 in ethylene signaling pathway. In the present study, we provided data showing that the Arabidopsis nucleoporin NUP160 and NUP96 participate in ethylene signaling-related mRNAs nucleocytoplasmic transport. The Arabidopsis nucleoporin mutants (nup160, nup96-1, nup96-2) exhibited enhanced ethylene sensitivity. Nuclear qRT-PCR analysis and poly(A)-mRNA in situ hybridization showed that the nucleoporin mutants affected the nucleocytoplasmic transport of all the examined mRNAs, including the ethylene signaling-related mRNAs such as ETR2, ERS1, ERS2, EIN4, CTR1, EIN2, and EIN3. Transcriptome analysis of the nucleoporin mutants provided clues suggesting that the nucleoporin NUP160 and NUP96 may participate in ethylene signaling via various molecular mechanisms. These observations significantly advance our understanding of the regulatory mechanisms of nucleoporin proteins in ethylene signaling and ethylene response.
拟南芥核孔蛋白通过控制mRNA的核质运输参与乙烯信号转导的调控。mRNA在细胞核和细胞质之间的双向运输由核孔复合体（NPC）控制。在高等植物中，NPC至少包含30种核孔蛋白。拟南芥核孔蛋白参与多种生物学过程，如病原体相互作用、结瘤、冷响应、开花和激素信号转导。然而，关于核孔蛋白NUP160和NUP96在乙烯信号通路中的调控功能知之甚少。在本研究中，我们提供的数据表明，拟南芥核孔蛋白NUP160和NUP96参与乙烯信号相关mRNA的核质运输。拟南芥核孔蛋白突变体（nup160、nup96-1、nup96-2）表现出增强的乙烯敏感性。细胞核定量逆转录聚合酶链反应分析和聚腺苷酸mRNA原位杂交表明，核孔蛋白突变体影响所有检测mRNA的核质运输，包括乙烯信号相关的mRNA，如ETR2、ERS1、ERS2、EIN4、CTR1、EIN2和EIN3。核孔蛋白突变体的转录组分析提供了线索，表明核孔蛋白NUP160和NUP96可能通过多种分子机制参与乙烯信号转导。这些观察结果显著推进了我们对核孔蛋白在乙烯信号转导和乙烯反应调控机制的理解。
