Chongsa-Nguan M, Chaicumpa W, Kalambaheti T, Surachedchai Y, Thanungkul B
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1987 Mar;18(1):33-8.
Specific antibodies to V. cholerae lipopolysaccharide (LPS), cell-bound haemagglutinin (CHA) and toxin (CT) in the intestinal lavages of healthy Thais and Thai cholera patients during the convalescence period were determined by enzyme-linked immunosorbent assay. Only IgM and IgA specific antibodies were detectable in the specimens. All of the persons who were just recovered from cholera had IgA anti-CT and IgA anti-LPS and 82.4% had IgA anti-CHA. The IgA anti-CT, anti-LPS and anti-CHA were detected also in the gut fluids of 70.6%, 94.1% and 88.2%, respectively, of the healthy controls. The mean levels of the IgA antibodies of all specificities between the two groups of individuals were not different. However, the IgM anti-CT and IgM anti-LPS of the cholera patients increased during the convalescence period. The levels, therefore, were significantly higher than those of the controls. The ratios of IgA anti-CT: IgM anti-CT and IgA anti-LPS: IgM anti-LPS among the patients were 2.93:1 and 2.02:1, respectively while those of the controls were 10:1 and 34:1, respectively. IgA antibodies predominated in the lavages of both groups of the individuals.
采用酶联免疫吸附测定法，对健康泰国人和泰国霍乱患者恢复期肠道灌洗液中霍乱弧菌脂多糖（LPS）、细胞结合血凝素（CHA）和毒素（CT）的特异性抗体进行了检测。标本中仅可检测到IgM和IgA特异性抗体。所有刚从霍乱中康复的人都有IgA抗CT和IgA抗LPS，82.4%的人有IgA抗CHA。在健康对照者的肠液中，分别有70.6%、94.1%和88.2%检测到了IgA抗CT、抗LPS和抗CHA。两组个体之间所有特异性的IgA抗体平均水平没有差异。然而，霍乱患者的IgM抗CT和IgM抗LPS在恢复期有所增加。因此，其水平显著高于对照组。患者中IgA抗CT:IgM抗CT和IgA抗LPS:IgM抗LPS的比值分别为2.93:1和2.02:1，而对照组分别为10:1和34:1。两组个体的灌洗液中均以IgA抗体为主。
