Debnath Samir C, Ghosh Amrita
St. John's Research and Development Centre, Agriculture and Agri-Food Canada, St. John's, NL, Canada.
Department of Biology, Memorial University of Newfoundland, St. John's, NL, Canada.
Front Plant Sci. 2022 Dec 19;13:1042726. doi: 10.3389/fpls.2022.1042726. eCollection 2022.
Berry crops, a nutrient powerhouse for antioxidant properties, have long been enjoyed as a health-promoting delicious food. Significant progress has been achieved for the propagation of berry crops using tissue culture techniques. Although bioreactor micropropagation has been developed as a cost-effective propagation technology for berry crops, genetic stability can be a problem for commercial micropropagation that can be monitored at morphological, biochemical, and molecular levels. Somaclonal variations, both genetic and epigenetic, in tissue culture regenerants are influenced by different factors, such as donor genotype, explant type and origin, chimeral tissues, culture media type, concentration and combination of plant growth regulators, and culture conditions and period. Tissue culture regenerants in berry crops show increased vegetative growth, rhizome production, and berry yield, containing higher antioxidant activity in fruits and leaves that might be due to epigenetic variation. The present review provides an in-depth study on various aspects of phenotypic variation in micropropagated berry plants and the epigenetic effects on these variations along with the role of DNA methylation, to fill the existing gap in literature.
浆果作物作为富含抗氧化特性的营养宝库，长期以来一直被视为一种促进健康的美味食品。利用组织培养技术繁殖浆果作物已取得显著进展。尽管生物反应器微繁殖已发展成为一种用于浆果作物的具有成本效益的繁殖技术，但遗传稳定性可能是商业微繁殖中的一个问题，可在形态、生化和分子水平上进行监测。组织培养再生植株中的体细胞克隆变异，包括遗传变异和表观遗传变异，受到不同因素的影响，如供体基因型、外植体类型和来源、嵌合组织、培养基类型、植物生长调节剂的浓度和组合，以及培养条件和培养周期。浆果作物的组织培养再生植株表现出营养生长增加、根茎产量提高和浆果产量增加，果实和叶片中含有较高的抗氧化活性，这可能归因于表观遗传变异。本综述对微繁殖浆果植物表型变异的各个方面以及这些变异的表观遗传效应以及DNA甲基化的作用进行了深入研究，以填补现有文献中的空白。
