Toro-Mendoza Jhoan, Maio Lucia, Gallego Marta, Otto Ferdinand, Schulz Florian, Parak Wolfgang J, Sanchez-Cano Carlos, Coluzza Ivan
CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo de Miramón 182, 20014Donostia-San Sebastián, Spain.
Universität Hamburg, Luruper Chaussee 149, 22607Hamburg, Germany.
ACS Nano. 2023 Jan 5. doi: 10.1021/acsnano.2c05682.
Nanoparticles (NPs) and other engineered nanomaterials have great potential as nanodrugs or nanomedical devices for biomedical applications. However, the adsorption of proteins in blood circulation or similar physiological fluids can significantly alter the surface properties and therapeutic response induced by most nanomaterials. For example, interaction with proteins can change the bloodstream circulation time and availability of therapeutic NPs or hinder the accumulation in their desired target organs. Proteins can also trigger or prevent agglomeration. By combining experimental and computational approaches, we have developed NPs carrying polyethylene glycol (PEG) polymeric coatings that mimic the surface charge distribution of proteins typically found in blood, which are known to show low aggregation under normal blood conditions. Here, we show that NPs with coatings based on apoferritin or human serum albumin display better antifouling properties and weaker protein interaction compared to similar NPs carrying conventional PEG polymeric coatings.
纳米颗粒（NPs）和其他工程纳米材料作为用于生物医学应用的纳米药物或纳米医疗设备具有巨大潜力。然而，纳米材料在血液循环或类似生理流体中吸附蛋白质会显著改变其表面性质以及大多数纳米材料所诱导的治疗反应。例如，与蛋白质的相互作用会改变治疗性纳米颗粒的血液循环时间和可用性，或阻碍其在目标器官中的蓄积。蛋白质还能引发或阻止团聚。通过结合实验和计算方法，我们开发了带有聚乙二醇（PEG）聚合物涂层的纳米颗粒，该涂层模拟了血液中常见蛋白质的表面电荷分布，已知在正常血液条件下这种分布显示出低聚集性。在此，我们表明，与带有传统PEG聚合物涂层的类似纳米颗粒相比，基于脱铁铁蛋白或人血清白蛋白涂层的纳米颗粒表现出更好的抗污性能和较弱的蛋白质相互作用。
