Lyon Neuroscience Research Center, TIGER Team, Bron, France.
Lyon 1 University, CNRS UMR 5292, Inserm U1028, Villeurbanne, France.
CNS Neurosci Ther. 2023 Mar;29(3):760-770. doi: 10.1111/cns.13977. Epub 2023 Jan 5.
The function of cannabinoid receptor type 2 (CB2R), mainly expressed by leukocytes, has long been limited to its peripheral immunomodulatory role. However, the use of CB2R-specific ligands and the availability of CB2R-Knock Out mice revealed that it could play a functional role in the CNS not only under physiological but also under pathological conditions. A direct effect on the nervous system emerged when CB2R mRNA was detected in neural tissues. However, accurate mapping of CB2R protein expression in the nervous system is still lacking, partly because of the lack of specificity of antibodies available. This review examines the regions and cells of the nervous system where CB2R protein is most likely present by cross-referencing mRNA and protein data published to date. Of the many antibodies developed to target CB2R, only a few have partially passed specificity tests and detected CB2R in the CNS. Efforts must be continued to support the development of more specific and better validated antibodies in each of the species in which CB2R protein is sought or needs to be quantified.
大麻素受体 2 型（CB2R）的功能，主要由白细胞表达，其外周免疫调节作用早已被确定。然而，使用 CB2R 特异性配体和 CB2R 敲除小鼠揭示了它不仅在生理条件下，而且在病理条件下都可以在中枢神经系统中发挥功能作用。当在神经组织中检测到 CB2R mRNA 时，出现了对神经系统的直接影响。然而，由于缺乏特异性抗体，CB2R 蛋白在神经系统中的准确表达图谱仍然缺乏。这篇综述通过交叉引用迄今为止发表的 mRNA 和蛋白质数据，检查了 CB2R 蛋白最有可能存在的神经系统区域和细胞。在为靶向 CB2R 而开发的众多抗体中，只有少数通过了部分特异性测试，并在中枢神经系统中检测到了 CB2R。必须继续努力支持在每个需要寻找或需要定量 CB2R 蛋白的物种中开发更具特异性和更好验证的抗体。
