Reise Markus, Kranz Stefan, Guellmar André, Wyrwa Ralf, Rosenbaum Tobias, Weisser Jürgen, Jurke Aileen, Schnabelrauch Matthias, Heyder Markus, Watts David C, Sigusch Bernd W
Department of Conservative Dentistry and Periodontology, Jena University Hospital, An der alten Post 4, 07743 Jena, Germany.
Department of Conservative Dentistry and Periodontology, Jena University Hospital, An der alten Post 4, 07743 Jena, Germany.
Dent Mater. 2023 Jan;39(1):132-139. doi: 10.1016/j.dental.2022.12.008. Epub 2023 Jan 4.
The aim of the present study was to prepare resorbable polylactide fibers for periodontitis treatment using coaxial electrospinning to optimize the release of metronidazole (MNA) by reducing the initial burst effect.
Poly(L-lactide-co-D,L-lactide) (PLA) fibers mats with different distributions of metronidazole (MNA) were manufactured by coaxial electrospinning (COAX). By COAX spinning the central core of the fiber was enriched with 40% MNA (m/m), while the sheath of the fiber consisted of PLA only (test group). In contrast, fibers of the control group were prepared by conventional electrospinning with the same amount of MNA but with a homogenous drug distribution (HDD - homogenously distributed drug). The release of MNA was determined by analyzing aliquots from the fiber mats using UV-VIS spectroscopy. Agar diffusion tests were carried out to determine the antibacterial effect on periodontopathogenic bacteria. Biocompatibility was tested in direct contact to human gingival fibroblasts (HGF) for two days.
The COAX mats showed a retarded drug release compared to the conventional HDD fibers. After 24 h, 64% of total MNA was released cumulatively from the COAX fibers while 90% of the MNA was released from the HDD fibers (controls). The antibacterial effect of COAX fibers was significantly higher after 24 h compared to the HDD fibers. Cell cultivation revealed significant higher numbers of vital cells among the COAX mats.
COAX fibers showed improved sustained MNA release compared to conventional fibers and can be seen as potential drug delivery systems in local periodontitis treatment.
本研究的目的是使用同轴静电纺丝制备用于治疗牙周炎的可吸收聚丙交酯纤维，通过减少初始突释效应来优化甲硝唑（MNA）的释放。
通过同轴静电纺丝（COAX）制造具有不同甲硝唑（MNA）分布的聚（L-丙交酯-co-D，L-丙交酯）（PLA）纤维垫。通过COAX纺丝，纤维的中心核富含40%的MNA（质量/质量），而纤维的鞘仅由PLA组成（试验组）。相比之下，对照组的纤维通过传统静电纺丝制备，MNA用量相同但药物分布均匀（HDD - 药物均匀分布）。通过使用紫外可见光谱分析纤维垫的等分试样来测定MNA的释放。进行琼脂扩散试验以确定对牙周病原菌的抗菌作用。在与人类牙龈成纤维细胞（HGF）直接接触两天的情况下测试生物相容性。
与传统的HDD纤维相比，COAX纤维垫显示出药物释放延迟。24小时后，COAX纤维累计释放了64%的总MNA，而HDD纤维（对照组）释放了90%的MNA。24小时后，COAX纤维的抗菌效果明显高于HDD纤维。细胞培养显示COAX纤维垫中活细胞数量明显更多。
与传统纤维相比，COAX纤维显示出改善的MNA持续释放，可被视为局部牙周炎治疗中的潜在药物递送系统。
