Sigusch Bernd W, Reise Markus, Kranz Stefan, Beck Julius, Wagner Kerstin, Guellmar André, Heyder Markus
Department of Conservative Dentistry and Periodontology, Center of Dental Medicine, Jena University Hospitals, 07743 Jena, Germany.
Innovent e.V., 07745 Jena, Germany.
Pharmaceutics. 2025 Jun 24;17(7):821. doi: 10.3390/pharmaceutics17070821.
The effect of fusidic acid on oral bacteria, especially on Gram- negative periodontopathogenic species, has not yet been investigated. This in vitro study aimed to analyze the antibacterial effect of fusidic acid alone and as an active component in electrospun poly(L-lactide-co-D/L-lactide) fiber fleeces. Minimal inhibitory concentrations (MIC) of fusidic acid and metronidazole (control) were determined for various oral bacteria. Eluates were collected from electrospun poly(L-lactide-co-D/L-lactide) fiber fleeces loaded with 10 and 20 wt% fusidic acid over a period of 28 d. Antibacterial activity was analyzed by means of a microdilution assay. Cytotoxicity was observed toward human gingival fibroblasts (HGFs). All tested Gram-positive and Gram-negative oral bacteria were susceptible to fusidic acid. The lowest MIC was observed for (MIC < 0.062 µg/mL). Compared to the antibacterial activity of metronidazole, that of was suppressed by significant lower fusidic acid concentrations ( < 0.01). The eluates obtained from electrospun poly(L-lactide-co-D/L-lactide) fiber fleeces inhibited the growth of , , , and over a course of 28 days. The largest inhibition zones were detected for in case of the 20 wt% concentrations. The eluates were not cytotoxic toward HGFs. It was shown that fusidic acid has significant antibacterial potential. The results of the present investigation suggest that fusidic acid alone or delivered by electrospun fiber fleeces might be attractive for controlling oral pathogenic bacteria.
夫西地酸对口腔细菌，尤其是革兰氏阴性牙周病原菌的作用尚未得到研究。本体外研究旨在分析夫西地酸单独以及作为电纺聚（L-丙交酯-co-D/L-丙交酯）纤维毡中的活性成分的抗菌效果。测定了夫西地酸和甲硝唑（对照）对多种口腔细菌的最低抑菌浓度（MIC）。在28天的时间里，从负载有10%和20%（重量）夫西地酸的电纺聚（L-丙交酯-co-D/L-丙交酯）纤维毡中收集洗脱液。通过微量稀释试验分析抗菌活性。观察了对人牙龈成纤维细胞（HGFs）的细胞毒性。所有测试的革兰氏阳性和革兰氏阴性口腔细菌均对夫西地酸敏感。观察到对[具体细菌名称未给出]的最低MIC（MIC<0.062μg/mL）。与甲硝唑的抗菌活性相比，夫西地酸在显著更低的浓度下（<0.01）就能抑制[具体细菌名称未给出]的抗菌活性。从电纺聚（L-丙交酯-co-D/L-丙交酯）纤维毡获得的洗脱液在28天的过程中抑制了[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]的生长。在20%（重量）浓度的情况下，对[具体细菌名称未给出]检测到最大的抑菌圈。洗脱液对HGFs没有细胞毒性。结果表明夫西地酸具有显著的抗菌潜力。本研究结果表明，单独的夫西地酸或通过电纺纤维毡递送的夫西地酸可能对控制口腔病原菌具有吸引力。
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