Bioinformatics Centre, North-Eastern Hill University, Shillong, Meghalaya, India.
Informatics and Big Data, CSIR-Institute of Genomics and Integrative Biology, Delhi, India.
BMC Immunol. 2023 Jan 5;24(1):1. doi: 10.1186/s12865-022-00535-y.
Continuous attempts have been made to pinpoint candidate vaccine molecules and evaluate their effectiveness in order to commercialise such vaccines for the treatment of tropical fascioliasis in livestock. The pathophysiology of fascioliasis can be related to liver damage brought on by immature flukes that migrate and feed, as well as immunological reactions to chemicals produced by the parasites and alarm signals brought on by tissue damage. Future research should, in our opinion, concentrate on the biology of invasive parasites and the resulting immune responses, particularly in the early stages of infection. The goal of the current study was to use the calcium-binding proteins from F. gigantica to create a multi-epitope subunit vaccine. The adjuvant, B-cell epitopes, CTL epitopes, and HTL epitopes that make up the vaccine construct are all connected by certain linkers. The antigenicity, allergenicity, and physiochemical properties of the vaccine construct were examined. The vaccine construct was docked with toll-like receptor 2, and simulations of the molecular dynamics of the complex's stability, interaction, and dynamics were run. After performing in silico cloning and immunosimulation, it was discovered that the construct was suitable for further investigation. New vaccination technologies and adjuvant development are advancing our food safety procedures since vaccines are seen as safe and are accepted by the user community. This research is also applicable to the F. hepatica system.
为了将此类疫苗商业化用于治疗家畜的热带片形吸虫病，人们一直在不断努力确定候选疫苗分子，并评估其有效性。片形吸虫病的病理生理学可以与不成熟的片形吸虫迁移和进食引起的肝损伤以及对寄生虫产生的化学物质的免疫反应和组织损伤引起的警报信号有关。我们认为，未来的研究应集中在侵袭性寄生虫的生物学和由此产生的免疫反应上，特别是在感染的早期阶段。本研究的目的是使用 F. gigantica 的钙结合蛋白来构建一种多表位亚单位疫苗。疫苗构建体由佐剂、B 细胞表位、CTL 表位和 HTL 表位组成，这些表位通过某些接头连接在一起。对疫苗构建体的抗原性、变应原性和物理化学性质进行了检测。对接 TLR2 并对复合物稳定性、相互作用和动力学的分子动力学进行模拟。在进行了计算机克隆和免疫模拟后，发现该构建体适合进一步研究。由于疫苗被认为是安全的，并被用户社区所接受，因此新的疫苗技术和佐剂的开发正在推进我们的食品安全程序。这项研究也适用于 F. hepatica 系统。
