多价疫苗对绵羊肝片吸虫感染的疗效。
Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep.
机构信息
Animal Health Department (Parasitology and Parasitic Diseases), Faculty of Veterinary Medicine, University of Córdoba, Sanidad Animal Building, Rabanales Campus, Córdoba, Spain.
Department of Anatomy, Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, Sanidad Animal Building, Rabanales Campus, Córdoba, Spain.
出版信息
Vet Res. 2021 Jan 28;52(1):13. doi: 10.1186/s13567-021-00895-0.
In this work we report the protection found in a vaccination trial performed in sheep with two different vaccines composed each one by a cocktail of antigens (rCL1, rPrx, rHDM and rLAP) formulated in two different adjuvants (Montanide ISA 61 VG (G1) and Alhydrogel(G2)). The parameters of protection tested were fluke burden, faecal egg count and evaluation of hepatic lesions. In vaccinated group 1 we found a significant decrease in fluke burden in comparison to both unimmunised and infected control group (37.2%; p = 0.002) and to vaccinated group 2 (Alhydrogel) (27.08%; p = 0.016). The lower fluke burden found in G1 was accompanied by a decrease in egg output of 28.71% in comparison with the infected control group. Additionally, gross hepatic lesions found in vaccine 1 group showed a significant decrease (p = 0.03) in comparison with unimmunised-infected group. The serological study showed the highest level for both IgG1 and IgG2 in animals from group 1. All these data support the hypothesis of protection found in vaccine 1 group.
在这项工作中，我们报告了在绵羊中进行的一项疫苗接种试验中的保护作用，该试验使用两种不同的疫苗，每种疫苗都由抗原（rCL1、rPrx、rHDM 和 rLAP）鸡尾酒组成，并用两种不同的佐剂（Montanide ISA 61 VG（G1）和 Alhydrogel（G2））配制。测试的保护参数是吸虫负荷、粪便卵计数和肝损伤评估。在接种组 1 中，与未免疫和感染对照组（37.2%；p=0.002）以及接种组 2（Alhydrogel）（27.08%；p=0.016）相比，我们发现吸虫负荷显著降低。在 G1 中发现的较低的吸虫负荷伴随着与感染对照组相比，卵产量降低了 28.71%。此外，疫苗 1 组的肝脏大体病变与未免疫感染组相比，显著减少（p=0.03）。血清学研究显示，第 1 组动物的 IgG1 和 IgG2 水平均最高。所有这些数据都支持在疫苗 1 组中发现的保护作用的假设。
Zotero 插件