Bordbar Hossein, Yahyavi Seyedeh-Saeedeh, Noorafshan Ali, Aliabadi Elham, Naseh Maryam
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Zand Ave., Shiraz, 71348-45794, Iran.
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Basic Clin Androl. 2023 Jan 6;33(1):1. doi: 10.1186/s12610-022-00174-8.
Bisphenol A (BPA) is one of the most widely used synthetic chemicals worldwide. BPA as an endocrine disruptor affects the reproductive systems through estrogenic and antiandrogenic proprieties. Resveratrol (RES) as a natural polyphenol and potent antioxidant exhibits protective effects against reproductive toxicity by inhibiting of oxidative stress. 48 male rats were divided into eight groups (n=6), including CONTROL, OLIVE OIL (0.5 ml/ day), Carboxy methylcellulose (CMC) (1 ml of 10 g/l), RES (100mg/kg/day), low dose of BPA (25 mg/kg/day), high dose of BPA (50 mg/kg/day), low dose of BPA + RES, and high dose of BPA + RES. All treatments were done orally per day for 56 days. At the end of the 8th week, blood samples were collected for hormone assays. Then, the sperm parameters were analyzed, and the left testis was removed for stereological study.
We showed a significant decrease in sperm parameters in the low and high doses of BPA groups compared to control groups (P<0.05). The volume of testicular components as well as the diameter and length of seminiferous tubules significantly reduced (11-64 %), and the total number of the testicular cell types decreased (34-67 %) on average in the low and high doses of BPA groups. Moreover, serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone hormones concentration showed a significant reduction in both doses of BPA groups (P<0.01). Nonetheless, treatment with RES could ameliorate all the above-mentioned changes in the low and high doses of BPA groups (P<0.05).
RES could prevent BPA-induced testicular structural changes and sperm quality via improving gonadotropin hormones and testosterone levels.
双酚A（BPA）是全球使用最广泛的合成化学品之一。BPA作为一种内分泌干扰物，通过雌激素和抗雄激素特性影响生殖系统。白藜芦醇（RES）作为一种天然多酚和强效抗氧化剂，通过抑制氧化应激对生殖毒性具有保护作用。将48只雄性大鼠分为八组（n = 6），包括对照组、橄榄油组（0.5毫升/天）、羧甲基纤维素（CMC）组（1毫升10克/升）、RES组（100毫克/千克/天）、低剂量BPA组（25毫克/千克/天）、高剂量BPA组（50毫克/千克/天）、低剂量BPA + RES组和高剂量BPA + RES组。所有处理均每天经口进行，持续56天。在第8周结束时，采集血样进行激素检测。然后，分析精子参数，并取出左侧睾丸进行体视学研究。
我们发现，与对照组相比，低剂量和高剂量BPA组的精子参数显著降低（P<0.05）。低剂量和高剂量BPA组的睾丸成分体积以及曲细精管的直径和长度均显著减少（11 - 64%），睾丸细胞类型总数平均减少（34 - 67%）。此外，BPA组两个剂量的血清促卵泡激素（FSH）、黄体生成素（LH）和睾酮激素浓度均显著降低（P<0.01）。然而，RES处理可改善低剂量和高剂量BPA组的上述所有变化（P<0.05）。
RES可通过提高促性腺激素和睾酮水平来预防BPA诱导的睾丸结构变化和精子质量下降。
