Lee Sungwoo, Jung Insub, Lee Soohyun, Lee Jaewon, Oh Myeong Jin, Park Minsun, Haddadnezhad MohammadNavid, Park Woocheol, Park Sungho
Department of Chemistry, Sungkyunkwan University, Suwon 16419, South Korea.
Institute of Basic Science, Sungkyunkwan University, Suwon 16419, South Korea.
Nanoscale Horiz. 2023 Jan 30;8(2):185-194. doi: 10.1039/d2nh00529h.
Here, we report a highly sensitive and reliable surface enhanced Raman scattering (SERS)-based immunoassay using bimetallic alloy Ag@Au hollow dual-rim nanorings (DRNs) where two hollow nanorings with different diameters are concentrically overlapped and connected by thin metal ligaments, forming circular hot-zones in the intra-nanogaps between the inner and outer rims. Pt DRNs were first prepared, and then Ag was deposited on the surface of the Pt skeleton, followed by Au coating, resulting in alloy Ag@Au hollow DRNs. The chemical stability of Au and the high optical properties of Ag are incorporated into a single entity, Ag@Au hollow DRNs, enabling strong single-particle SERS activity and biocompatibility through surface modification with thiol-containing functionalities. When Ag@Au hollow DRNs were utilized as nanoprobes for detecting human chorionic gonadotropin (HCG) hormone through a SERS-based immunoassay, a very low limit of detection of 10 pM with high reliability was achieved, strongly indicating their advantage as ultrasensitive SERS nanoprobes.
在此，我们报道了一种基于表面增强拉曼散射（SERS）的高灵敏度且可靠的免疫分析方法，该方法使用双金属合金Ag@Au空心双环纳米环（DRNs），其中两个不同直径的空心纳米环同心重叠并通过细金属韧带连接，在内环和外环之间的纳米间隙内形成圆形热点区域。首先制备Pt DRNs，然后在Pt骨架表面沉积Ag，接着进行Au包覆，从而得到合金Ag@Au空心DRNs。Au的化学稳定性和Ag的高光光学性质被整合到单一实体Ag@Au空心DRNs中，通过用含硫醇官能团进行表面修饰，实现了强大的单颗粒SERS活性和生物相容性。当将Ag@Au空心DRNs用作纳米探针，通过基于SERS的免疫分析检测人绒毛膜促性腺激素（HCG）时，实现了低至10 pM的检测限且可靠性高，有力地表明了它们作为超灵敏SERS纳米探针的优势。
