Wang Zhi, Ma Weichao, Gao Zisen, Zhao Zhihan, Du Baojiang, Wei Junqing, Jiang Dun, Lan Kuibo, Chen Ruibing, Yan Shanchun, Qin Guoxuan
School of Microelectronics, Tianjin Key Laboratory of Imaging and Sensing Microelectronic Technology, Tianjin University, Tianjin 300072, P. R. China.
College of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, P. R. China.
ACS Sens. 2023 Jan 27;8(1):363-371. doi: 10.1021/acssensors.2c02384. Epub 2023 Jan 6.
The cotton bollworm, (), causes damage to a wide range of cultivated crops and is one of the pests with the greatest economic importance for global agriculture. Currently, the detection of is based on manual sampling. A low limit of detection (LOD), convenient, and real-time monitoring method is urgently needed for its early warning and efficient management. Here, we characterized the amino acid sequence in the sex pheromone receptors (SPRs) recognizing the pheromone components of by three-dimensional (3D) modeling and molecular docking. Next, sex pheromone receptor-derived peptides (SPRPs) were synthesized and conjugated to nanotubes by chemical connection. The modified nanotubes were used to fabricate a sensor capable of real-time monitoring of gaseous sex pheromone compounds with a low LOD (∼10 ppb for Z11-16:Ald) and selectivity, and the sensor was able to detect a single live . Furthermore, the developed biosensor allowed direct monitoring of the pheromone release dynamics by female and showed that the release was instantly reduced in response to light. Here, we report the first demonstration of a biosensing method for detecting gaseous sex pheromones and live . The findings show the great potential of the SPRP sensor for broad applications in insect biology study and infestation monitoring.
棉铃虫（Helicoverpa armigera）对多种栽培作物造成损害，是全球农业中具有最大经济重要性的害虫之一。目前，棉铃虫的检测基于人工采样。迫切需要一种低检测限（LOD）、便捷且实时的监测方法用于其早期预警和有效管理。在此，我们通过三维（3D）建模和分子对接对识别棉铃虫性信息素成分的性信息素受体（SPR）中的氨基酸序列进行了表征。接下来，合成了性信息素受体衍生肽（SPRPs），并通过化学连接将其与纳米管偶联。修饰后的纳米管被用于制造一种能够以低检测限（对于Z11-16:Ald约为10 ppb）实时监测气态性信息素化合物且具有选择性的传感器，该传感器能够检测单个活体棉铃虫。此外，所开发的生物传感器能够直接监测雌性棉铃虫的性信息素释放动态，并表明其释放会因光照而立即减少。在此，我们首次展示了一种用于检测气态性信息素和活体棉铃虫的生物传感方法。这些发现表明SPRPs传感器在昆虫生物学研究和虫害监测的广泛应用中具有巨大潜力。
