Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), NIH, Bethesda, MD 20892, USA.
Genomic Technology Section, NIAMS, Bethesda, MD 20892, USA.
STAR Protoc. 2023 Mar 17;4(1):102001. doi: 10.1016/j.xpro.2022.102001. Epub 2023 Jan 5.
Mouse embryonic stem cells (mESCs) can be directed to acquire cell-lineage-specific genetic programs and phenotypes by stepwise exposure to defined factors, allowing the development of in vitro models for studying disease and tissue generation. In this protocol, we describe the use of cultured mESCs to generate presomitic-like mesoderm cells undergoing further specification towards myogenic progenitors (MPs). Further, we describe here a procedure to obtain, dissect, and fluorescence-activated cell sorting (FACS)-isolate somitic cells from genetically labeled Pax7; Rosa26 embryos. For complete details on the use and execution of this protocol, please refer to Khateb et al..
小鼠胚胎干细胞（mESCs）可以通过逐步暴露于特定因子来指导获得细胞谱系特异性的遗传程序和表型，从而为研究疾病和组织生成开发体外模型。在本方案中，我们描述了使用培养的 mESCs 生成类体节中胚层细胞，这些细胞进一步特化为肌前体细胞（MPs）。此外，我们还描述了一种从遗传标记的 Pax7;Rosa26 胚胎中获取、解剖和荧光激活细胞分选（FACS）分离体节细胞的方法。有关此方案使用和执行的完整详细信息，请参阅 Khateb 等人的研究。
