Department of Chemistry and Chemical and Biomedical Engineering, Interim Chair, Mechanical and Industrial Engineering, University of New Haven, West Haven, CT, 06516, USA.
Department of Chemistry and Chemical and Biomedical Engineering, University of New Haven, West Haven, CT, 06516, USA.
Macromol Biosci. 2023 Apr;23(4):e2200479. doi: 10.1002/mabi.202200479. Epub 2023 Jan 31.
The artificial lung has provided life-saving support for pulmonary disease patients and recently afforded patients with severe cases of COVID-19 better prognostic outcomes. While it addresses a critical medical need, reducing the risk of clotting inside the device remains challenging. Herein, a two-step surface coating process of the lung circuit using Zwitterionic polysulfobetaine methacrylate is evaluated for its nonspecific protein antifouling activity. It is hypothesized that similarly applied coatings on materials integrated (IT) or nonintegrated (NIT) into the circuit will yield similar antifouling activity. The effects of human plasma preconditioned with nitric oxide-loaded liposome on platelet (plt) fouling are also evaluated. Fibrinogen antifouling activities in coated fibers are similar in the IT and NIT groups. It however decreases in coated polycarbonate (PC) in the IT group. Also, plt antifouling activity in coated fibers is similar in the IT and NIT groups and is lower in coated PC and Tygon in the IT group compared to the NIT group. Coating process optimization in the IT lung circuit may help address difference in the coating appearance of outer and inner fiber bundle fibers, and the NO-liposome significantly reduces (86%) plt fouling on fibers indicating its potential use for blood anticoagulation.
人工肺为肺部疾病患者提供了救生支持，最近为严重 COVID-19 患者提供了更好的预后结果。虽然它满足了关键的医疗需求，但降低设备内部凝血的风险仍然具有挑战性。在此，评估了两步式使用两性离子聚磺丁基甜菜碱甲基丙烯酸盐对肺回路进行表面涂层的方法，以评估其非特异性蛋白质抗污活性。假设应用于集成（IT）或非集成（NIT）到回路中的材料的类似涂层将产生类似的抗污活性。还评估了用载有一氧化氮的脂质体预处理的人血浆对血小板（plt）污染的影响。涂层纤维中的纤维蛋白原抗污活性在 IT 组和 NIT 组中相似。但是，在 IT 组中，涂覆的聚碳酸酯（PC）中的纤维蛋白原抗污活性降低。此外，在 IT 组中，涂层纤维中的 plt 抗污活性在 IT 组和 NIT 组中相似，而在 IT 组中，涂层 PC 和 Tygon 的 plt 抗污活性低于 NIT 组。IT 肺回路中的涂层工艺优化可能有助于解决外纤维束和内纤维束涂层外观的差异，并且一氧化氮脂质体可显著降低纤维上的 plt 污染（86%），表明其在血液抗凝方面具有潜在用途。
