利用 RNA-Seq 技术调控玉米叶片角度的研究

Study on Regulating Leaf Angle in Maize by RNA-Seq.

机构信息

College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.

出版信息

Int J Mol Sci. 2022 Dec 22;24(1):189. doi: 10.3390/ijms24010189.

DOI:10.3390/ijms24010189

PMID:36613631

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9820655/

Abstract

Ubiquitin/proteasome-mediated proteolysis (UPP) plays a crucial role in almost all aspects of plant growth and development, proteasome subunit RPN10 mediates ubiquitination substrate recognition in the UPP process. The recognition pathway of ubiquitinated UPP substrate is different in different species, which indicates that the mechanism and function of are different in different species. However, the homologous in maize has not been studied. In this study, the changing of leaf angle and gene expression in leaves in maize wild-type B73 and mutant under exogenous brassinosteroids (BRs) were investigated. The regulation effect of BR on the leaf angle of was significantly stronger than that of B73. Transcriptome analysis showed that among the differentially expressed genes, CRE1, A-ARR and SnRK2 were significantly up-regulated, and PP2C, BRI1 AUX/IAA, JAZ and MYC2 were significantly down-regulated. This study revealed the regulation mechanism of on maize leaf angle and provided a promising gene resource for maize breeding.

摘要

泛素/蛋白酶体介导的蛋白降解（UPP）在植物生长和发育的几乎所有方面都起着至关重要的作用，蛋白酶体亚基 RPN10 在 UPP 过程中介导泛素化底物的识别。不同物种中泛素化 UPP 底物的识别途径不同，这表明不同物种中的机制和功能不同。然而，玉米中的同源物尚未得到研究。在这项研究中，研究了玉米野生型 B73 和突变体在外源油菜素内酯（BRs）下叶片角度和叶片中基因表达的变化。BR 对的叶片角度的调节作用明显强于 B73。转录组分析表明，在差异表达基因中，CRE1、A-ARR 和 SnRK2 显著上调，而 PP2C、BRI1 AUX/IAA、JAZ 和 MYC2 显著下调。这项研究揭示了对玉米叶片角度的调节机制，为玉米育种提供了有前途的基因资源。