Rossier B C
J Membr Biol. 1978;40 Spec No:187-97. doi: 10.1007/BF02026005.
Recent data describing the effects of aldosterone on the induction of messenger RNA (= mRNA) and ribosomal RNA (= rRNA) are reviewed. In the urinary bladder of the toad, aldosterone induces a few specific polyadenylated mRNAs (= poly(A)(+)mRNA) during the latent period, i.e., 30 to 60 min after hormone addition. Later i.e., 90 to 240 min after aldosterone addition, 18S and 28S cytoplasmic rRNA subunits are also induced. The effect of poly(A)(+)mRNA is mineralocorticoid-specific and correlates well with the aldosterone-dependent Na+ transport. Actinomycin D which inhibits both poly(A)(+)mRNA and nonpolyadenylated mRNA (= POLY(A)(-)mRNA) totally abolishes the response to aldosterone on Na+ transport. 3'deoxyadenosine (cordycepin), which inhibits poly(A)(+)mRNA but not poly(A)(-)mRNA, only inhibits 50 to 60% of the physiological response. These differential effects suggest that an intact poly(A)(-)mRNA pathway is also an important factor in mediating the action of aldosterone. In contrast, 3'deoxycytidine, which inhibits rRNA but not mRNA, does not impair the mineralocorticoid response, at least during the first 3 hr of aldosterone action.
本文综述了近期有关醛固酮对信使核糖核酸（=mRNA）和核糖体核糖核酸（=rRNA）诱导作用的相关数据。在蟾蜍的膀胱中，醛固酮在潜伏期（即添加激素后30至60分钟）诱导产生一些特定的多聚腺苷酸化mRNA（=poly(A)(+)mRNA）。之后，即在添加醛固酮后90至240分钟，18S和28S细胞质rRNA亚基也被诱导产生。poly(A)(+)mRNA的这种作用具有盐皮质激素特异性，且与醛固酮依赖的钠转运密切相关。放线菌素D可同时抑制poly(A)(+)mRNA和非多聚腺苷酸化mRNA（=POLY(A)(-)mRNA），它能完全消除醛固酮对钠转运的反应。3'-脱氧腺苷（虫草素）可抑制poly(A)(+)mRNA但不抑制poly(A)(-)mRNA，它仅抑制50%至60%的生理反应。这些差异效应表明，完整的poly(A)(-)mRNA途径也是介导醛固酮作用的一个重要因素。相比之下，3'-脱氧胞苷可抑制rRNA但不抑制mRNA，至少在醛固酮作用的最初3小时内，它不会损害盐皮质激素反应。
