Unit of Training and Research in Life and Earth Sciences, Department of Biochemistry-Microbiology, Nazi Boni University, Bobo-Dioulasso 01 BP 1091, Burkina Faso.
Laboratory of Research and Teaching in Animal Health and Biotechnology, Nazi Boni University, Bobo-Dioulasso 01 BP 1091, Burkina Faso.
Molecules. 2023 Jan 2;28(1):393. doi: 10.3390/molecules28010393.
and are widely used by herbalists to cure diabetes mellitus. The aim of this study is to investigate the inhibitory potential of aqueous and various organic solvent fractions from both plants and some isolated compounds against advanced glycation end-products (AGEs). For this purpose, an in vitro BSA-fructose glycation model was used to evaluate the inhibition of AGE formation. Furthermore, the effects of the fractions on mouse fibroblast (NIH-3T3) and human hepatocyte (HepG2) survival were evaluated. The leaf, stem, and root fractions of both plants exhibited significant inhibition of AGEs formation. The IC values appeared to be less than 250 µg/mL; however, all fractions presented no adverse effects on NIH-3T3 up to 500 µg/mL. Otherwise, our phytochemical investigation afforded the isolation of a secoiridoid from the genus named secoiridoid glucoside sweroside (), along with three known quinovic acid glycosides: quinovic acid-3---d-glucopyranoside (), quinovic acid-3---d-6-deoxy-glucopyranoside, 28---d-glucopyranosyl ester (), and quinovic acid 3---l-rhamnopyranosyl-(4→1)--d-glucopyranoside (). In particular, - are compounds which have not previously been described in roots. However, the isolated compounds did not exhibit AGE inhibitory activity. Further investigation on these potent antiglycation fractions may allow for the isolation of new antidiabetic drug candidates.
和 被草药学家广泛用于治疗糖尿病。本研究旨在研究这两种植物的水相和各种有机溶剂相以及一些分离化合物对晚期糖基化终产物 (AGEs) 的抑制潜力。为此，使用体外 BSA-果糖糖化模型来评估 AGE 形成的抑制作用。此外，还评估了各馏分对小鼠成纤维细胞 (NIH-3T3) 和人肝细胞 (HepG2) 存活的影响。两种植物的叶、茎和根馏分均表现出对 AGEs 形成的显著抑制作用。IC 值似乎小于 250 µg/mL；然而，所有馏分在高达 500 µg/mL 的浓度下对 NIH-3T3 均无不良影响。此外，我们的植物化学研究从 属中分离出一种名为 secoiridoid glucoside sweroside () 的裂环环烯醚萜，以及三种已知的奎诺酸糖苷：奎诺酸-3---D-吡喃葡萄糖苷 ()、奎诺酸-3---D-6-脱氧吡喃葡萄糖苷、28---D-吡喃葡萄糖苷酯 () 和奎诺酸 3---L-鼠李吡喃糖基-(4→1)--D-吡喃葡萄糖苷 ()。特别是 - 是以前在 根中未描述过的化合物。然而，分离出的化合物没有表现出 AGE 抑制活性。对这些具有强大糖化抑制作用的馏分的进一步研究可能会分离出新型抗糖尿病药物候选物。
