MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
Heredity (Edinb). 2011 Apr;106(4):520-30. doi: 10.1038/hdy.2010.93. Epub 2010 Aug 4.
Retrotransposons are both major generators of genetic diversity and tools for detecting the genomic changes associated with their activity because they create large and stable insertions in the genome. After the demonstration that retrotransposons are ubiquitous, active and abundant in plant genomes, various marker systems were developed to exploit polymorphisms in retrotransposon insertion patterns. These have found applications ranging from the mapping of genes responsible for particular traits and the management of backcrossing programs to analysis of population structure and diversity of wild species. This review provides an insight into the spectrum of retrotransposon-based marker systems developed for plant species and evaluates the contributions of retrotransposon markers to the analysis of population diversity in plants.
逆转录转座子既是遗传多样性的主要产生者,也是检测与其活性相关的基因组变化的工具,因为它们在基因组中产生大而稳定的插入。在证明逆转录转座子在植物基因组中普遍存在、活跃且丰富之后,开发了各种标记系统来利用逆转录转座子插入模式的多态性。这些标记系统已应用于从负责特定性状的基因定位到回交计划管理,再到分析野生物种的种群结构和多样性等各个领域。本文综述了为植物物种开发的基于逆转录转座子的标记系统的范围,并评估了逆转录转座子标记在植物种群多样性分析中的贡献。