Iijima H, Ishii M, Yamauchi K, Chao C L, Kimura K, Shimura S, Shindoh Y, Inoue H, Mue S, Takishima T
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Am Rev Respir Dis. 1987 Oct;136(4):922-9. doi: 10.1164/ajrccm/136.4.922.
To elucidate the mechanisms of late asthmatic response (LAR) observed in asthmatic subjects, we have developed an animal model of LAR using guinea pigs. Fifty guinea pigs were immunized with a mixture of Ascaris suum extract and aluminum hydroxide and then challenged with an inhalation of Ascaris suum extract without anesthesia. Twenty of the 50 guinea pigs showed a dual asthmatic response in which the LAR occurred 3 to 6 h after immediate asthmatic response (IAR). Histologic studies by rapid freezing with liquid nitrogen or bronchoalveolar lavage (BAL) were performed in 14 of these 20 guinea pigs with LAR and compared with those in 10 of 18 guinea pigs with only IAR, 10 control guinea pigs, and 10 nonimmunized but challenged guinea pigs. Both the percentage and the absolute number of neutrophils in the BAL fluid of the guinea pigs with LAR were significantly greater than those of the control guinea pigs (p less than 0.02) and than those of the nonimmunized but challenged guinea pigs (p less than 0.02). However, that of guinea pigs with LAR was not significantly different from that of guinea pigs with only IAR. On the other hand, histologic examination showed that eosinophil infiltration within the airway walls of the guinea pigs with LAR was more prominent than that of the guinea pigs with only IAR, and showed that there was no significant difference in neutrophil infiltration within the airway walls between the guinea pigs with LAR and the animals with only IAR. Contraction of airway (bronchus, bronchiole) smooth muscle, submucosal edema, and mucus in airway lumen were also observed in LAR.(ABSTRACT TRUNCATED AT 250 WORDS)
为阐明哮喘患者中观察到的迟发性哮喘反应（LAR）的机制，我们利用豚鼠建立了LAR动物模型。50只豚鼠用猪蛔虫提取物和氢氧化铝混合物进行免疫，然后在未麻醉的情况下吸入猪蛔虫提取物进行激发。50只豚鼠中有20只出现双相哮喘反应，其中LAR在速发性哮喘反应（IAR）后3至6小时出现。对这20只出现LAR的豚鼠中的14只进行了液氮快速冷冻或支气管肺泡灌洗（BAL）的组织学研究，并与18只仅出现IAR的豚鼠中的10只、10只对照豚鼠以及10只未免疫但接受激发的豚鼠进行比较。出现LAR的豚鼠BAL液中中性粒细胞的百分比和绝对数量均显著高于对照豚鼠（p<0.02）以及未免疫但接受激发的豚鼠（p<0.02）。然而，出现LAR的豚鼠与仅出现IAR的豚鼠相比无显著差异。另一方面，组织学检查显示，出现LAR的豚鼠气道壁内嗜酸性粒细胞浸润比仅出现IAR的豚鼠更明显，且出现LAR的豚鼠与仅出现IAR的豚鼠气道壁内中性粒细胞浸润无显著差异。在LAR中还观察到气道（支气管、细支气管）平滑肌收缩、黏膜下水肿和气道腔内黏液。（摘要截断于250字）
