State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, People's Republic of China.
Microbiol Spectr. 2023 Feb 14;11(1):e0190622. doi: 10.1128/spectrum.01906-22. Epub 2023 Jan 10.
The gut microbiota is known to play a role in regulating host metabolism, yet the mechanisms underlying this regulation are not well elucidated. Our study aimed to characterize the differences in gut microbiota compositions and their roles in iron absorption between wild-type (WT) and / double-gene-knockout (DKO) weaned piglets. A total of 58 samples along the entire digestive tract were analyzed for microbial community using 16S rRNA gene sequencing. The colonic microbiota and their metabolites were determined by metagenomic sequencing and untargeted liquid chromatography-mass spectrometry (LC-MS), respectively. Our results showed that no alterations in microbial community structure and composition were observed between DKO and WT weaned piglets, with the exception of colonic microbiota. Interestingly, the DKO piglets had selectively increased the relative abundance of the genus belonging to the Neisseriaceae family and decreased the genus abundance. Functional capacity analysis showed that organic acid metabolism was enriched in the colon in DKO piglets. In addition, the DKO piglets showed increased iron levels in important tissues compared with WT piglets without any pathological changes. Pearson's correlation coefficient indicated that the specific bacteria such as and genus played a key role in host iron absorption. Moreover, the iron levels had significantly (0.05) positive correlation with microbial metabolites, particularly carboxylic acids and their derivatives, which might increase iron absorption by preventing iron precipitation. Overall, this study reveals an interaction between colonic microbiota and host metabolism and has potential significance for alleviating piglet iron deficiency. Iron deficiency is a major risk factor for iron deficiency anemia, which is among the most common nutritional disorders in piglets. However, it remains unclear how the gut microbiota interacts with host iron absorption. The current report provides the first insight into iron absorption-microbiome connection in / double knockout piglets. The present results showed that carboxylic acids and their derivatives contributed to the absorption of nonheme iron by preventing ferric iron precipitation.
肠道微生物群被认为在调节宿主代谢中发挥作用，但这种调节的机制尚不清楚。我们的研究旨在描述野生型（WT）和/双重基因敲除（DKO）断奶仔猪肠道微生物群落组成的差异及其在铁吸收中的作用。使用 16S rRNA 基因测序对整个消化道的 58 个样本进行微生物群落分析。通过宏基因组测序和非靶向液相色谱-质谱（LC-MS）分别测定结肠微生物群及其代谢物。我们的结果表明，除了结肠微生物群外，DKO 和 WT 断奶仔猪的微生物群落结构和组成没有变化。有趣的是，DKO 仔猪选择性地增加了属于奈瑟菌科的属的相对丰度，降低了属的丰度。功能能力分析表明，有机酸代谢在 DKO 仔猪的结肠中富集。此外，与 WT 仔猪相比，DKO 仔猪在重要组织中的铁含量增加，而没有任何病理变化。Pearson 相关系数表明，特定细菌，如属和属，在宿主铁吸收中发挥关键作用。此外，铁水平与微生物代谢物呈显著正相关（0.05），特别是羧酸及其衍生物，它们通过防止铁沉淀来增加铁的吸收。总体而言，这项研究揭示了结肠微生物群与宿主代谢之间的相互作用，对缓解仔猪缺铁症具有潜在意义。缺铁是仔猪缺铁性贫血的主要危险因素，缺铁性贫血是仔猪最常见的营养障碍之一。然而，肠道微生物群如何与宿主铁吸收相互作用尚不清楚。本报告首次提供了 / 双重基因敲除仔猪铁吸收-微生物组联系的见解。目前的结果表明，羧酸及其衍生物通过防止三价铁沉淀来促进非血红素铁的吸收。
