Department of Stomatology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, Zhejiang Province, China.
Medicine (Baltimore). 2022 Dec 9;101(49):e32119. doi: 10.1097/MD.0000000000032119.
Head and neck squamous cell carcinoma (HNSCC) is one of the most common malignancies worldwide, because its discovery time is in the late stage of the disease, so it is important to develop HNSCC biomarkers to achieve the purpose of early detection and treatment. Fatty acid desaturase 3 (FADS3), the third member of the FADS family, is involved in sphingolipid biosynthesis. Here, we for the first time investigated FADS3 expression in HNSCC, as well as its potential biological function, prognostic value and its impact on the immune system. In this study, we used bioinformatics for gene expression analysis, clinicopathological analysis, enrichment analysis, and immune infiltration analysis of The Cancer Genome Atlas (TCGA) datasets. Statistical analysis was done using R. Tumor IMmune Estimation Resource (TIMER) and CIBERSORT were used to analyze the effect of FADS3 on immune responses in HNSCC. Gene Expression Profiling Interactive Analysis (GEPIA), Kaplan-Meier (KM) survival analysis, and the Human Protein Atlas (HPA) data were used to validate the results from bioinformatics analysis. Our findings indicate that FADS3 influences HNSCC prognosis. High expression of FADS3 is related to higher lymphatic metastasis, histologic grade, and lymphovascular invasion. Gene set enrichment analysis (GSEA) revealed that FADS3 is related to inhibition of amino acid metabolism. CIBERSORT analysis showed high FADS3 expression correlates with reduced levels of B cells. FADS3 is a marker of HNSCC, and high expression of FADS3 is associated with poor prognosis of HNSCC.
头颈部鳞状细胞癌（HNSCC）是全球最常见的恶性肿瘤之一，由于其发现时间处于疾病晚期，因此开发 HNSCC 生物标志物以实现早期检测和治疗的目的非常重要。脂肪酸去饱和酶 3（FADS3）是 FADS 家族的第三个成员，参与鞘脂生物合成。在这里，我们首次研究了 HNSCC 中的 FADS3 表达及其潜在的生物学功能、预后价值及其对免疫系统的影响。在这项研究中，我们使用了生物信息学方法对 TCGA 数据集进行基因表达分析、临床病理分析、富集分析和免疫浸润分析。使用 R 进行统计分析。使用 Tumor IMmune Estimation Resource（TIMER）和 CIBERSORT 分析 FADS3 对 HNSCC 免疫反应的影响。使用基因表达谱交互分析（GEPIA）、Kaplan-Meier（KM）生存分析和人类蛋白质图谱（HPA）数据来验证生物信息学分析的结果。我们的研究结果表明，FADS3 影响 HNSCC 的预后。FADS3 的高表达与更高的淋巴转移、组织学分级和淋巴血管侵犯有关。基因集富集分析（GSEA）显示 FADS3 与抑制氨基酸代谢有关。CIBERSORT 分析表明，FADS3 高表达与 B 细胞水平降低有关。FADS3 是 HNSCC 的标志物，FADS3 的高表达与 HNSCC 的不良预后相关。
