Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur-176061, Himachal Pradesh, India; Department of Microbiology, Guru Nanak Dev University, Amritsar-143005, Punjab, India.
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur-176061, Himachal Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India.
Gene. 2023 Mar 20;857:147178. doi: 10.1016/j.gene.2023.147178. Epub 2023 Jan 7.
Janthinobacterium from cold niches has been studied broadly for bioactive violacein production. However, reports on the atypical red-pigmented Janthinobacterium strains are shallow. The bioactive red prodigiosin pigment has immense pharmacological significance, including antioxidant, antimicrobial and anticancer potential. Here, we report the first complete genome of a prodigiosin-producing Janthinobacterium sp. ERMR3:09 from Sikkim Himalaya in an attempt to elucidate its cold adaptation and prodigiosin biosynthesis. Nanopore sequencing and Flye assembly of the ERMR3:09 genome resulted in a single contig of 6,262,330 bp size and 62.26% GC content. Phylogenomic analysis and genome indices indicate that ERMR3:09 is a potentially novel species of the genus Janthinobacterium. The multicopy cold-responsive genes and gene upregulation under cold stress denoted its cold adaptation mechanisms. Genome analysis identified the unique genes, gene cluster and pathway for prodigiosin biosynthesis in ERMR3:09. Considering the notable antioxidant activity, it can be the next powerhouse of bioactive prodigiosin production.
从寒冷生境中分离得到的詹氏菌属被广泛研究用于生物活性紫堇色素的生产。然而，关于非典型红色詹氏菌属菌株的报道还很肤浅。生物活性红色灵菌红素具有巨大的药理学意义，包括抗氧化、抗菌和抗癌潜力。在这里，我们报告了来自喜马拉雅山锡金的产灵菌红素詹氏菌属 sp. ERMR3:09 的第一个完整基因组，试图阐明其对寒冷的适应和灵菌红素生物合成。对 ERMR3:09 基因组进行纳米孔测序和 Flye 组装，得到一个大小为 6,262,330 bp、GC 含量为 62.26%的单一连续序列。系统发育基因组学分析和基因组指标表明，ERMR3:09 是詹氏菌属的一个潜在新种。多拷贝的冷响应基因和冷胁迫下的基因上调表明了其对寒冷的适应机制。基因组分析确定了 ERMR3:09 中灵菌红素生物合成的独特基因、基因簇和途径。考虑到其显著的抗氧化活性，它可能是生物活性灵菌红素生产的下一个强大力量。
