Li Dan, Lu Jiamin, Hu Zhuoyi, Liang Jiajian, Lin Shibin
Department of Ophthalmology, Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.
Photobiomodul Photomed Laser Surg. 2023 Jan;41(1):17-25. doi: 10.1089/photob.2022.0063.
To assess the effects of intense pulsed light (IPL) on oxidative stress (OS) in perennial allergic conjunctivitis (PAC). IPL treatment has been proven effective for dry eye disease (DED). However, we have observed that, after IPL treatment, some patients with DED combined with allergic conjunctivitis (AC), an immune response condition in which excessive OS causes and exacerbates inflammatory damage, not only show an improvement in eye dryness, but also their AC-related eye itching is relieved. The mechanism by which IPL inhibits allergic reactions is not clear. Five patients with moderate-to-severe PAC were given two IPL treatments on the periorbital skin with a 2-week interval. Visual analog scale (VAS) scores and signs of AC, including eye redness and conjunctival follicles, were assessed before the first treatment (day 1) and 2 weeks after the second treatment (day 30). Tears were also collected at the same time, and lipid oxidation (LPO) metabolite analysis was performed using liquid chromatography tandem mass spectrometry (LC-MS/MS) to investigate the effects of IPL on OS response. The average VAS score significantly decreased with treatment (30.2 for day 1, 10.6 for day 30; < 0.001). The general signs of PAC showed no difference ( > 0.05). LPO metabolite analysis revealed that 17,18-diHETE, which is an oxidation product of eicosapentaenoic acid, and 13-OXoODE, which is an oxidation product of linoleic acid, are significantly downregulated after IPL treatment. The photothermal effect of IPL attenuates OS in PAC, and this seems to be one of the mechanisms by which IPL treatment improves PAC. Clinical Trial Registration number: ChiCTR1900022202.
评估强脉冲光（IPL）对常年性变应性结膜炎（PAC）氧化应激（OS）的影响。IPL治疗已被证明对干眼症（DED）有效。然而，我们观察到，在IPL治疗后，一些患有DED合并变应性结膜炎（AC）的患者，AC是一种免疫反应性疾病，其中过度的OS会导致并加剧炎症损伤，这些患者不仅眼干症状有所改善，而且与AC相关的眼部瘙痒也得到缓解。IPL抑制过敏反应的机制尚不清楚。对5例中重度PAC患者的眶周皮肤进行两次IPL治疗，间隔2周。在第一次治疗前（第1天）和第二次治疗后2周（第30天）评估视觉模拟量表（VAS）评分以及AC的体征，包括眼红和结膜滤泡。同时收集泪液，采用液相色谱串联质谱（LC-MS/MS）进行脂质氧化（LPO）代谢物分析，以研究IPL对OS反应的影响。治疗后平均VAS评分显著降低（第1天为30.2，第30天为10.6；<0.001）。PAC的总体体征无差异（>0.05）。LPO代谢物分析显示，二十碳五烯酸的氧化产物17,18-二氢二十碳四烯酸（17,18-diHETE）和亚油酸的氧化产物13-氧代十八碳二烯酸（13-OXoODE）在IPL治疗后显著下调。IPL的光热效应减弱了PAC中的OS，这似乎是IPL治疗改善PAC的机制之一。临床试验注册号：ChiCTR1900022202。
