An Mengya, Liang Ruoxi, Chen Yanliu, Zhang Jinhua, Wang Xiuqing, Li Xing, Qu Guohua, Liang Junfeng
Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
J Fungi (Basel). 2025 Jan 15;11(1):62. doi: 10.3390/jof11010062.
is a well-known edible and medicinal fungus with significant economic value. However, the available whole-genome information is lacking for this species. The chromosome-scale reference genome (Monop) and two haploid genomes (Hap1 and Hap2) of , each assembled into 11 pseudochromosomes, were constructed using Illumina, PacBio-HiFi long-read sequencing, and Hi-C technology. The Monop had a size of 36.54 Mb, with 10,251 predicted protein-coding genes and including 433 carbohydrate-active enzyme genes, 385 cytochrome P450 enzyme genes, and 42 gene clusters related to secondary metabolite synthesis. Phylogenetic and collinearity analysis revealed a close evolutionary relationship between and in the core Phallales clade. Hap1 and Hap2 had sizes of 35.46 Mb and 36.11 Mb, respectively. Collinear relationships were not observed for 15.38% of the genes in the two haplotypes. Hap1 had 256 unique genes, and Hap2 had 370 unique genes. Our analysis of the genome provides insights into the genetic basis of the mechanisms underlying the metabolic effects of bioactive substances and will aid ongoing breeding efforts and studies of genetic mechanisms.
是一种具有重要经济价值的著名食药用真菌。然而，该物种缺乏可用的全基因组信息。利用Illumina、PacBio-HiFi长读长测序和Hi-C技术构建了的染色体水平参考基因组（Monop）和两个单倍体基因组（Hap1和Hap2），每个基因组都组装成11条假染色体。Monop的大小为36.54 Mb，有10251个预测的蛋白质编码基因，包括433个碳水化合物活性酶基因、385个细胞色素P450酶基因和42个与次生代谢物合成相关的基因簇。系统发育和共线性分析揭示了与核心鬼笔目分支中的在进化上有密切关系。Hap1和Hap2的大小分别为35.46 Mb和36.11 Mb。两种单倍型中15.38%的基因未观察到共线关系。Hap1有256个独特基因，Hap2有370个独特基因。我们对基因组的分析为生物活性物质代谢效应潜在机制的遗传基础提供了见解，并将有助于正在进行的育种工作和遗传机制研究。
