National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Sci Data. 2023 May 9;10(1):259. doi: 10.1038/s41597-023-02171-6.
Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.
尼泊尔岩高兰（Coriaria nepalensis Wall.）（岩高兰科）是一种固氮灌木，它与放线菌弗兰克氏菌形成根瘤。据报道，尼泊尔岩高兰的油和提取物具有抑菌和杀虫作用，尼泊尔岩高兰的树皮是一种有价值的单宁资源。在这里，我们结合 PacBio HiFi 测序和 Hi-C 支架技术，为尼泊尔岩高兰生成了一个单倍型解析的染色体级别的基因组组装。该基因组组装大小约为 620Mb，contig N50 为 11Mb，总组装序列的 99.9%锚定在 40 个假染色体上。我们预测了 60862 个蛋白质编码基因，其中 99.5%从数据库中注释。我们进一步鉴定了 939 个 tRNA、7297 个 rRNA 和 982 个 ncRNA。尼泊尔岩高兰的染色体级基因组有望成为理解与弗兰克氏菌共生结瘤、毒性和单宁生物合成的遗传基础的重要资源。
Zotero 插件
