McLellan L I, Hayes J D
University Department of Clinical Chemistry, Royal Infirmary, Edinburgh, Scotland, U.K.
Biochem J. 1987 Jul 15;245(2):399-406. doi: 10.1042/bj2450399.
Hepatic glutathione S-transferase isoenzyme content has been investigated in both sexes of three inbred strains of mice (DBA/2, C3H/He, C57BL6). A polypeptide (Mr 24,800), which is immunologically related to Yf purified from rat lung, was found to be expressed as a major form in all male mouse livers but represented only a minor enzyme form in female mouse liver. Glutathione S-transferases comprising subunits with molecular masses of 25,800 (Ya) or 26,400 (Yb) were present in males and females of the three strains under investigation. Cytosolic isoenzymes from all strains and sexes were purified to apparent homogeneity and no significant inter-strain differences in the properties of the individual forms were observed. In addition, no differences were detected in the microsomal glutathione S-transferase content of the different strains or sexes.
对三种近交系小鼠（DBA/2、C3H/He、C57BL6）的雌雄个体的肝脏谷胱甘肽S-转移酶同工酶含量进行了研究。发现一种与从大鼠肺中纯化的Yf具有免疫相关性的多肽（分子量24,800）在所有雄性小鼠肝脏中以主要形式表达，但在雌性小鼠肝脏中仅代表次要的酶形式。在所研究的三个品系的雄性和雌性中均存在由分子量为25,800（Ya）或26,400（Yb）的亚基组成的谷胱甘肽S-转移酶。所有品系和性别的胞质同工酶均被纯化至表观均一，且未观察到各个形式的性质存在显著的品系间差异。此外，不同品系或性别的微粒体谷胱甘肽S-转移酶含量未检测到差异。
