Singh S V, Saunders M O, Moller P C, Haber B, Awasthi Y C
Comp Biochem Physiol B. 1987;86(1):73-81. doi: 10.1016/0305-0491(87)90177-5.
Six forms of glutathione S-transferase (GST) designated as GST 9.3, GST 7.5, GST 6.6, GST 6.1, GST 5.7 and GST 4.9 have been purified to homogeneity from rat brain. All GST isoenzymes of rat brain are apparent homodimers of one of the three type subunits, Ya, Yb, or Yc. More than 60% of total GST activity of rat brain GST activity is associated with the isoenzymes containing only the Yb type of subunits. In these respects brain GST isoenzymes differ from those of lung and liver. The Ya, Yb, and Yc type subunits of brain GST are immunologically similar to the corresponding subunits of liver and lung GST. The isoelectric points and kinetic properties of the Yb type subunit dimers in brain are strikingly different from those of the Yb type dimers present among liver GST isoenzymes indicating subtle differences between these subunits of brain and liver.
已从大鼠脑中纯化出六种谷胱甘肽S-转移酶（GST），分别命名为GST 9.3、GST 7.5、GST 6.6、GST 6.1、GST 5.7和GST 4.9，均达到同质。大鼠脑内所有GST同工酶显然都是三种亚基类型（Ya、Yb或Yc）之一的同型二聚体。大鼠脑GST活性中超过60%与仅含Yb型亚基的同工酶相关。在这些方面，脑GST同工酶与肺和肝中的不同。脑GST的Ya、Yb和Yc型亚基在免疫上与肝和肺GST的相应亚基相似。脑中Yb型亚基二聚体的等电点和动力学特性与肝GST同工酶中存在的Yb型二聚体显著不同，表明脑和肝的这些亚基之间存在细微差异。
