Joensuu H, Klemi P J, Eerola E
Department of Radiotherapy, University Central Hospital of Turku, Finland.
Anal Quant Cytol Histol. 1987 Aug;9(4):328-34.
The nuclear DNA content and the numbers of cells in the S and G2M phases of the cell cycle were determined by flow cytometry (FCM) in fine needle aspirates of 187 thyroid tumors to evaluate the diagnostic value of nuclear DNA content determination in combination with aspiration cytology. DNA aneuploidy was present in 4 of 5 follicular carcinomas, 2 of 3 anaplastic carcinomas, 5 of 15 excised follicular adenomas and 2 of 20 excised adenomatous goiters; all 7 papillary carcinomas and 4 lymphomas were diploid in the aspirate. Aneuploid carcinomas had easily distinguishable S and/or G2M phases, unlike the benign aneuploid tumors. None of the histologically benign tumors or the nonexcised tumors had greater than 6% S-phase cells, and only one benign tumor had greater than 9% G2M-phase cells. In contrast, all lymphomas had greater than 10% S-phase cells and four of seven papillary carcinomas had greater than 9% G2M-phase cells. The use of FCM determination in combination with fine needle aspiration biopsy cytology improved the diagnostic potential of the latter technique.
通过流式细胞术（FCM）测定了187例甲状腺肿瘤细针穿刺抽吸物中的核DNA含量以及细胞周期S期和G2M期的细胞数量，以评估核DNA含量测定联合细针穿刺细胞学检查的诊断价值。在5例滤泡癌中有4例、3例未分化癌中有2例、15例切除的滤泡性腺瘤中有5例以及20例切除的腺瘤性甲状腺肿中有2例存在DNA非整倍体；所有7例乳头状癌和4例淋巴瘤的穿刺抽吸物均为二倍体。与良性非整倍体肿瘤不同，非整倍体癌具有易于区分的S期和/或G2M期。组织学上为良性的肿瘤或未切除的肿瘤中，S期细胞均不超过6%，只有1例良性肿瘤的G2M期细胞超过9%。相比之下，所有淋巴瘤的S期细胞均超过10%，7例乳头状癌中有4例的G2M期细胞超过9%。将FCM测定与细针穿刺活检细胞学检查相结合提高了后一种技术的诊断潜力。
